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Gresens, J., Cook, K. (2006.8.29). Isolation and characterization of Df(3R)BSC198. 
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Date: Tue, 29 Aug 2006  15:37:30  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3R)BSC198
Cc: kaufmanXXXX, mdealXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX>
Isolation and characterization of Df(3R)BSC198
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC198 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}f02841 and P{XP}d04754. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
w[1118]; Dr[1]/TM6B, Tb[1] females crossed to P{hsFLP}1, y[1] 
w[1118]; PBac{WH}f02841/P{XP}d04754 males. The males were heat 
shocked as larvae as described in Parks et al. Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC198 from the segment of 
PBac{WH}f02841 to the left of its FRT site and the segment of 
P{XP}d04754 to the right of its FRT site. The cytological breakpoints 
of Df(3R)BSC198 predicted from the transposable element insertions 
sites using Release 4 coordinates are 84F13;85A2. It failed to 
complement l(3)84Fk[2] and stck[EY06672].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)