Isolation and characterization of Df(2L)BSC168
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC168 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG11030f07078 and P{XP}eIF-4ad06487. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG11030f07078/P{XP}eIF-4ad06487 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC168 from the segment of PBac{WH}CG11030f07078 to the left of its FRT site and the segment of P{XP}eIF-4ad06487 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC168 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 25F4;26B2. It failed to complement Vm26AbQJ42 and chic221.