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Christensen, S., Cook, K. (2006.8.30). Isolation and characterization of Df(2R)BSC199. 
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Date: Wed, 30 Aug 2006  15:12:35  -0400
To: flybase-updates@XXXX
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC199
Cc: kaufmanXXXX, Stacey Christensen <sjchristXXXX>, Jill Gresens <jgresensXXXX>, mdealXXXX
Isolation and characterization of Df(2R)BSC199
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC199 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d10427 and PBac{RB}Oda[e03814]. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d10427/PBac{RB}Oda[e03814] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.RB3}BSC199 from the segment 
of P{XP}d10427 to the left of its FRT site and the segment of 
PBac{RB}Oda[e03814] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(2R)BSC199 predicted from the transposable element insertions sites 
using Release 4 coordinates are 48D1;48E4. It failed to complement jeb[k05644].
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)