Isolation and characterization of Df(2L)BSC254
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC254 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04398 and P{XP}d09327. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}f04398/P{XP}d09327 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC254 from the segment of PBac{WH}f04398 to the left of its FRT site and the segment of P{XP}d09327 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC254 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 35B6;35C1. It failed to complement l(2)35Bbk11524a, l(2)35Bd10408, ck13 and vas1.