Reference Report

Reference
Citation	Christensen, S., Cook, K. (2007.5.8). Isolation and characterization of Df(2R)BSC305. (Export to RIS)
FlyBase ID	FBrf0199306
Publication Type	Personal communication to FlyBase
PubMed ID
PubMed Abstract
Text of Personal Communication	From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> To: flybase-updates@XXXXXXXXXXXXXXX Cc: mdealXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX>, kaufman@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(2R)BSC305 Date: Tue, 08 May 2007 08:46:35 -0400 (13:46 BST) Isolation and characterization of Df(2R)BSC305 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC305 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06853 and PBac{WH}fdl[f02041]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}d06853/PBac{WH}fdl[f02041] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC305 from the segment of P{XP}d06853 to the left of its FRT site and the segment of PBac{WH}fdl[f02041] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC305 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 49A4;49A10. It failed to complement Lac[BG01462] and Df(2R)BSC3. From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> To: flybase-updates@XXXXXXXXXXXXXXX Cc: mdealXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX>, kaufman@XXXXXXXXXXXXXXX Subject: Isolation and characterization of Df(2R)BSC305 Date: Tue, 08 May 2007 08:46:35 -0400 (13:46 BST) Isolation and characterization of Df(2R)BSC305 Stacey Christensen and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC305 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06853 and PBac{WH}fdl[f02041]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}d06853/PBac{WH}fdl[f02041] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC305 from the segment of P{XP}d06853 to the left of its FRT site and the segment of PBac{WH}fdl[f02041] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC305 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 49A4;49A10. It failed to complement Lac[BG01462] and Df(2R)BSC3.
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Language of Publication	English
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Data from Reference
Aberrations (2)
	Df(2R)BSC3 Df(2R)BSC305
Alleles (1)
	Lac^BG01462
Constructs (1)
	P+PBac{XP5.WH5}
Genes (1)
	Lac
Insertions (3)
	P{XP}CG8525^d06853 P+PBac{XP5.WH5}BSC305 PBac{WH}fdl^f02041