Reference Report

Reference
Citation	Kracklauer, M.P., Banks, S.M.L., Xie, X., Wu, Y., Fischer, J.A. (2007). Drosophila klaroid encodes a SUN domain protein required for Klarsicht localization to the nuclear envelope and nuclear migration in the eye. Fly 1(2): 75--85. (Export to RIS)
FlyBase ID	FBrf0200125
Publication Type	Research paper
PubMed ID	18820457
PubMed Abstract	KASH (Klarsicht/Anc-1/Syne homology) domain proteins are cytoskeleton-associated proteins localized uniquely to the outer nuclear membrane. Klarsicht is a KASH protein required for nuclear migration in differentiating cells of the Drosophila eye. The C-terminal KASH domain of Klarsicht resides in the perinuclear space, and the cytoplasmic moiety connects to the microtubule organizing center. In C. elegans and vertebrate cells, SUN (Sad1/UNC-84) domain proteins reside in the inner nuclear membrane and tether KASH proteins to the outer nuclear membrane. Is there a Drosophila SUN protein that performs a similar function, and if so, is it like Klarsicht, obviously essential for nuclear positioning only in the eye? Here, we identify Drosophila Klaroid, a SUN protein that tethers Klarsicht. klaroid loss-of-function mutants are indistinguishable phenotypically from klarsicht mutants. Remarkably, neither gene is essential for Drosophila viability or fertility, and even in klaroid klorsicht double mutants, the only obvious external morphological defect is rough eyes. In addition, we find that klaroid and klarsicht are required for nuclear migration in differentiating neurons and in non-neural cells. Finally, while perinuclear Klaroid is ubiquitous in the eye, Klarsicht expression is limited to differentiating cells and may be part of the trigger for apical nuclear migration.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Fly
Title	Fly
Publication Year	2007-
ISBN/ISSN	1933-6934 1933-6942
Data from Reference
Aberrations (2)
	Df(2R)Exel6050 Df(3L)emc-E12
Alleles (20)
	klar^marb-BP klar^marb-CD4 klar^{Scer\UAS.P\T.6.T:Hsap\MYC} klar^α.ro klar^{αKASH.Scer\UAS.T:Avic\GFP} koi^HRKO58.w koi^HRKO79.w koi^HRKO80.w koi^HRKO84.w koi^HRKO86.w koi^{HRKO.Scer\SceI.RS} koi^{Scer\UAS.T:Avic\GFP} koi^{Scer\UAS.T:Hsap\MYC} Lam⁰⁴⁶⁴³ Lam^{Scer\UAS.T:Avic\GFP} Scer\FLP1^hs.PP Scer\GAL4^elav.PLu Scer\SCEI^hs.PR Ube3a^{Scer\UAS.T:Avic\GFP} w⁺
Constructs (11)
	P{70FLP} P{70I-SceI} P{FRT(w⁺.koi^{HRKO.Scer\SceI.RS})} P{GAL4-elav.L} P{ro-klarα} P{UAS-As.gfp} P{UAS-klar.αKASH.GFP} P{UAS-koi.6Xmyc} P{UAS-koi.gfp} P{UAS-Lam.GFP} P{UASp-klar.6Xmyc}
Genes (15)
	Act5C Act42A ct elav futsch klar koi Lam Scer\FLP1 Scer\GAL4 Scer\SCEI spag4 T:Avic\GFP T:Hsap\MYC Ube3a
Natural transposons (1)
	P-element