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Citation
Osoegawa, K., Vessere, G.M., Shu, C.L., Hoskins, R.A., Abad, J.P., de Pablos, B., Villasante, A., de Jong, P.J. (2007). BAC clones generated from sheared DNA.  Genomics 89(2): 291--299.
FlyBase ID
FBrf0200134
Publication Type
Research paper
Abstract

BAC libraries generated from restriction-digested genomic DNA display representational bias and lack some sequences. To facilitate completion of genome projects, procedures have been developed to create BACs from DNA physically sheared to create fragments extending up to 200 kb. The DNA fragments were repaired to create blunt ends and ligated to a new BAC vector. This approach has been tested by generating BAC libraries from Drosophila DNA with insert lengths between 50 and 150 kb. The libraries lack chimeric clone problems as determined by mapping paired BAC-end sequences to the assembled fly genome sequence. The utility of "sheared" libraries was demonstrated by closure of a previous clone gap and by isolation of clones from telomeric regions, which were notably absent from previous Drosophila BAC libraries.

PubMed ID
PubMed Central ID
PMC1909752 (PMC) (EuropePMC)
Associated Information
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Secondary IDs
  • FBrf0194596
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Genomics
Title
Genomics
Publication Year
1987-
ISBN/ISSN
0888-7543
Data From Reference
Genes (4)
Datasets (5)