Reference Report

Reference
Citation	Ward, E.J., Zhou, X., Berg, C.A., Ruohola-Baker, H. (2006). Border of Notch activity establishes a boundary between the two dorsal appendage tube cell types. Dev. Biol. 297(2): 461--470. (Export to RIS)
FlyBase ID	FBrf0200192
Publication Type	Research paper
PubMed ID	16828735
PubMed Abstract	Boundaries establish and maintain separate populations of cells critical for organ formation. We show that Notch signaling establishes the boundary between two types of post-mitotic epithelial cells, the Rhomboid- and the Broad-positive cells. These cells will undergo morphogenetic movements to generate the two sides of a simple organ, the dorsal appendage tube of the Drosophila egg chamber. The boundary forms due to a difference in Notch levels in adjacent cells. The Notch expression pattern mimics the boundary; Notch levels are high in Rhomboid cells and low in Broad cells. Notch(-) mutant clones generate an ectopic boundary: ectopic Rhomboid cells arise in Notch(+) cells adjacent to the Notch(-) mutant cells but not further away from the clonal border. Pangolin, a component of the Wingless pathway, is required for Broad expression and for rhomboid repression. We further show that Broad represses rhomboid cell autonomously. Our data provide a foundation for understanding how a single row of Rhomboid cells arises adjacent to the Broad cells in the dorsal appendage primordia. Generating a boundary by the Notch pathway might constitute an evolutionarily conserved first step during organ formation in many tissues.
DOI	10.1016/j.ydbio.2006.05.021
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Secondary IDs	FBrf0193073
Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (14)
	br^npr-3 Dl::N^{ΔECN.Scer\UAS} Dl^RevF10 Dl^Scer\UAS.cJa Ecol\lacZ^rho.2.2 Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} N^55e11 N^l1N-ts1 N^Scer\UAS.cSa pan^ΔN.Scer\UAS Scer\GAL4^αTub84B.PL Ser^RX106 Su(H)⁸ Su(H)^del47
Constructs (7)
	P{Rho(ve)-lacZ.2.2} P{tubP-GAL4} P{UAS-Dl::N.ΔECN} P{UAS-Dl.J} P{UAS-mCD8::GFP.L} P{UAS-N.S} P{UAS-pan.dTCFΔN}
Genes (12)
	br Dl Dl::N Ecol\lacZ Fas3 Mmus\Cd8a N pan rho Scer\GAL4 Ser Su(H)
Insertions (3)
	P{UAS-Dl::N.ΔECN}B2a2 P{UAS-Dl.J}TJ1 P{UAS-pan.dTCFΔN}5