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Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(2L)BSC345. 
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2L)BSC345
Date: 	Mon, 29 Oct 2007  12:07:18  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2L)BSC345
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC345 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d03477 and PBac{RB}e02962. The deletion was 
isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d03477/PBac{RB}e02962 males crossed to 
w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat 
shocked as larvae as described in Parks et al., Nature Genetics 36: 
288-292, 2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC345 from the segment of P{XP}d03477 
to the left of its FRT site and the segment of PBac{RB}e02962 to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. with the substitution 
of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or 
RB3' minus primer in the Hybrid PCR protocol in the Supplementary 
Methods. Exelixis, Inc. determined the insertion site of P{XP}d03477 
to be at Release 3 genomic coordinate 13860811 on chromosome arm 2L. 
This corresponds to 34E1 on both the Release 3 and 5 genome maps. The 
predicted position of PBac{RB}e02962 on the Release 5 map is 34E5. 
Consequently, the cytological breakpoints of Df(2L)BSC345 are 
predicted to be 34E1;34E5. It failed to complement Df(2L)b87e25 and 
Df(2L)BSC252. Df(2L)FDD-0048357 is a synonym for Df(2L)BSC345.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    Aberrations (3)
    Insertions (3)
    Transgenic Constructs (1)