Isolation and characterization of Df(2L)BSC353
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC353 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Gpdhf02250 and P{XP}ade2d06349. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}Gpdhf02250/P{XP}ade2d06349 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC353 from the segment of PBac{WH}Gpdhf02250 to the left of its FRT site and the segment of P{XP}ade2d06349 to the right of its FRT site. The cytological breakpoints of Df(2L)BSC353 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 26A3;26B3. It failed to complement Vm26AbQJ42 and chic221. Df(2L)FDD-0289332 is a synonym for Df(2L)BSC353.