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Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(2R)BSC326. 
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FBrf0200235
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From: 	Kevin Cook <kcook@XXXX>
To: 	flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
Subject: 	Isolation and characterization of Df(2R)BSC326
Date: 	Mon, 29 Oct 2007  12:07:16  -0400  ( 16:07  GMT)
Isolation and characterization of Df(2R)BSC326
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC326 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}d04812 and PBac{WH}phtf[f01263]. The deletion 
was isolated as a chromosome lacking miniwhite markers in progeny of 
P{hsFLP}1, y[1] w[1118]; P{XP}d04812/PBac{WH}phtf[f01263] males 
crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males 
were heat shocked as larvae as described in Parks et al., Nature 
Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in 
preceding and succeeding generations maintained the original genetic 
background of the Exelixis insertion stocks (Thibault et al., Nature 
Genetics 36: 283-287, 2004; FBrf0175002). The recombination event 
generated the genetic element P+PBac{XP5.WH5}BSC326 from the segment 
of P{XP}d04812 to the left of its FRT site and the segment of 
PBac{WH}phtf[f01263] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
Exelixis, Inc. determined the insertion site of P{XP}d04812 to be at 
Release 3 genomic coordinate 1374527 on chromosome arm 2R. This 
corresponds to 42A14 on both the Release 3 and 5 genome maps. The 
predicted position of PBac{WH}phtf[f01263] on the Release 5 map is 
42C7 . Consequently, the cytological breakpoints of Df(2R)BSC326 are 
predicted to be 42A14;42C7. It failed to complement jing[01094] and 
Df(2R)ED1618.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX 
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (2)