Reference Report

Reference
Citation	Christensen, S., Gresens, J., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(3R)BSC316. (Export to RIS)
FlyBase ID	FBrf0200247
Publication Type	Personal communication to FlyBase
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Text of Personal Communication	To: flybase-updatesXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(3R)BSC316 Date: Mon, 29 Oct 2007 12:07:15 -0400 Isolation and characterization of Df(3R)BSC316 Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3R)BSC316 was isolated as a FLP recombinase-induced recombination event involving PBac{RB}tub[e03259] and P{XP}d09816. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6B females crossed to P{hsFLP}1, w[1118]; PBac{RB}tub[e03259]/P{XP}d09816 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC316 from the segment of PBac{RB}tub[e03259] to the left of its FRT site and the segment of P{XP}d09816 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(3R)BSC316 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 82A5;82B3. Df(3R)FDD-0201678 is a synonym for Df(3R)BSC316.
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Language of Publication	English
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Data from Reference
Aberrations (1)
	Df(3R)BSC316
Constructs (1)
	P+PBac{XP5.RB3}
Insertions (3)
	P{XP}d09816 P+PBac{XP5.RB3}BSC316 PBac{RB}tub^e03259