Reference Report

Reference
Citation	Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(3L)BSC401. (Export to RIS)
FlyBase ID	FBrf0200257
Publication Type	Personal communication to FlyBase
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Text of Personal Communication	To: flybase-updatesXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(2R)BSC401 Date: Mon, 29 Oct 2007 12:08:33 -0400 Isolation and characterization of Df(2R)BSC401 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC401 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG18327[f01465] and P{XP}d01851. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; PBac{WH}CG18327[f01465]/P{XP}d01851 males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC401 from the segment of PBac{WH}CG18327[f01465] to the left of its FRT site and the segment of P{XP}d01851 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC401 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 50E1;50E4. It failed to complement Sox15[KG09145]. Df(2R)FDD-0270175 is a synonym for Df(2R)BSC401.
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Language of Publication	English
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Aberrations (1)
	Df(2R)BSC401
Alleles (1)
	Sox15^KG09145
Constructs (1)
	P+PBac{XP5.WH5}
Genes (1)
	Sox15
Insertions (3)
	P{XP}d01851 P+PBac{XP5.WH5}BSC401 PBac{WH}CG18327^f01465