Isolation and characterization of Df(2R)BSC359
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC359 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Cbp53Ef00876 and P{XP}GstS1d07643. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}Cbp53Ef00876/P{XP}GstS1d07643 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC359 from the segment of PBac{WH}Cbp53Ef00876 to the left of its FRT site and the segment of P{XP}GstS1d07643 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC359 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 53E4;53F8. It failed to complement RhoGEF204291 and Df(2R)ED2751.