Isolation and characterization of Df(2R)BSC267 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC267 was isolated as a FLP recombinase-induced recombination event involving P{XP}sut1d07339 and PBac{WH}pdm3f00828. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}sut1d07339/PBac{WH}pdm3f00828 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC267 from the segment of P{XP}sut1d07339 to the left of its FRT site and the segment of PBac{WH}pdm3f00828 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC267 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 44A4;44C4. It failed to complement cul-4KG02900 and pnutXP. Df(2R)FDD-0089200 is a synonym for Df(2R)BSC267.