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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(2R)BSC399. (Export to RIS) | ||
| FlyBase ID | FBrf0200289 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
Date: Mon, 29 Oct 2007 12:07:21 -0400
To: flybase-updatesXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(2R)BSC399 Isolation and characterization of Df(2R)BSC399 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(2R)BSC399 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG5224[d01951] and PBac{RB}Hs3st-A[e00779]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y[1] w[1118]; P{XP}CG5224[d01951]/PBac{RB}Hs3st-A[e00779] males crossed to w[1118]; P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC399 from the segment of P{XP}CG5224[d01951] to the left of its FRT site and the segment of PBac{RB}Hs3st-A[e00779] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(2R)BSC399 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 55D1;55E10. It failed to complement Df(2R)PC4. Df(2R)BSC399 FDD-0029493 is a synonym for Df(2R)BSC399. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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| Aberrations (2)
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| Constructs (1)
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| Insertions (3)
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