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Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(3L)BSC367. 
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Date: Mon, 29 Oct 2007  12:07:20  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC367
Isolation and characterization of Df(3L)BSC367
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC367 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}Sk2[d05405] and PBac{WH}CG14962[f03417]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; P{XP}Sk2[d05405]/PBac{WH}CG14962[f03417] males. These 
males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC367 from the segment of P{XP}Sk2[d05405]to the left 
of its FRT site and the segment of PBac{WH}CG14962[f03417] to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC367 predicted from the Release 5 genomic 
coordinates of the transposable element insertion sites are 
63A3;63B8. Df(3L)BSC367 failed to complement rasp[m47]. 
Df(3L)FDD-0069207 is a synonym for Df(3L)BSC367.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (1)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)