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Reference Report
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| Citation | Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(3L)BSC379. (Export to RIS) | ||
| FlyBase ID | FBrf0200302 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
Date: Mon, 29 Oct 2007 12:09:35 -0400
To: flybase-updatesXXXXXXXXXXXXXXX, Stacey Christensen <sjchristXXXXXXXXXXXXXXX> From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: Isolation and characterization of Df(3L)BSC379 Isolation and characterization of Df(3L)BSC379 Stacey Christensen, Kimberley Cook and Kevin Cook Bloomington Stock Center Indiana University Df(3L)BSC379 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}GlcAT-P[f03130] and P{XP}chrb[d03716]. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, y[1] w[1118]; PBac{WH}GlcAT-P[f03130]/P{XP}chrb[d03716] males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC379 from the segment of PBac{WH}GlcAT-P[f03130]to the left of its FRT site and the segment of P{XP}chrb[d03716] to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC379 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 68B1;68C10. Df(3L)BSC379 failed to complement Df(3L)vin5 and Df(3L)ED4470. Df(3L)FDD-0310282 is a synonym for Df(3L)BSC379. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-856-1213 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-7005 kcook@XXXXXXXXXXXXXXX |
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| Language of Publication | English | ||
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| Aberrations (3)
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| Constructs (1)
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| Insertions (3)
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