Isolation and characterization of Df(3L)BSC379
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC379 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}GlcAT-Pf03130 and P{XP}chrbd03716. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}GlcAT-Pf03130/P{XP}chrbd03716 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC379 from the segment of PBac{WH}GlcAT-Pf03130to the left of its FRT site and the segment of P{XP}chrbd03716 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC379 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 68B1;68C10. Df(3L)BSC379 failed to complement Df(3L)vin5 and Df(3L)ED4470. Df(3L)FDD-0310282 is a synonym for Df(3L)BSC379.