Date: Mon, 29 Oct 2007  12:09:35  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(3L)BSC379
Isolation and characterization of Df(3L)BSC379
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC379 was isolated as a FLP recombinase-induced recombination 
event involving PBac{WH}GlcAT-P[f03130] and P{XP}chrb[d03716]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of w[1118]; Dr[1]/TM6C, Sb[1] females crossed to P{hsFLP}1, 
y[1] w[1118]; PBac{WH}GlcAT-P[f03130]/P{XP}chrb[d03716] males. These 
males were heat shocked as larvae as described in Parks et al., 
Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and 
crosses in preceding and succeeding generations maintained the 
original genetic background of the Exelixis insertion stocks 
(Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). 
The recombination event generated the genetic element 
P+PBac{XP5.WH5}BSC379 from the segment of PBac{WH}GlcAT-P[f03130]to 
the left of its FRT site and the segment of P{XP}chrb[d03716] to the 
right of its FRT site. Its presence was verified using the PCR 
methods and primers described in Parks et al. The cytological 
breakpoints of Df(3L)BSC379 predicted from the Release 5 genomic 
coordinates of the transposable element insertion sites are 
68B1;68C10. Df(3L)BSC379 failed to complement Df(3L)vin5 and 
Df(3L)ED4470. Df(3L)FDD-0310282 is a synonym for Df(3L)BSC379.
__________________________________________________________
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology           http://flystocks.bio.indiana.edu
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX