Isolation and characterization of Df(3R)BSC319
Stacey Christensen, Jill Gresens, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC319 was isolated as a FLP recombinase-induced recombination event involving P{XP}casd10725 and PBac{WH}f02632. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}casd10725/PBac{WH}f02632 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC319 from the segment of P{XP}casd10725 to the left of its FRT site and the segment of PBac{WH}f02632 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC319 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 83C1;83D3. Df(3R)BSC319 failed to complement Rm62EY06795 and Df(3R)Exel7284. Df(3R)FDD-0116475 is a synonym for Df(3R)BSC319.