Reference Report

Reference
Citation	Schittenhelm, R.B., Heeger, S., Althoff, F., Walter, A., Heidinann, S., Mechtler, K., Lehner, C.F. (2007). Spatial organization of a ubiquitous eukaryotic kinetochore protein network in Drosophila chromosomes. Chromosoma 116(4): 385--402. (Export to RIS)
FlyBase ID	FBrf0200315
Publication Type	Research paper
PubMed ID	17333235
PubMed Abstract	Chromosome segregation during meiosis and mitosis depends on the assembly of functional kinetochores within centromeric regions. Centromeric DNA and kinetochore proteins show surprisingly little sequence conservation despite their fundamental biological role. However, our identification in Drosophila melanogaster of the most diverged orthologs identified so far, which encode components of a kinetochore protein network including the Ndc80 and Mis complexes, further emphasizes the notion of a shared eukaryotic kinetochore design. To determine its spatial organization, we have analyzed by quantitative light microscopy hundreds of native chromosomes from transgenic Drosophila strains coexpressing combinations of red and green fluorescent fusion proteins, fully capable of providing the essential wild-type functions. Thereby, Cenp-A/Cid, Cenp-C, Mis12 and the Ndc80 complex were mapped along the inter sister kinetochore axis with a resolution below 10 nm. The C terminus of Cenp-C was found to be near but well separated from the innermost component Cenp-A/Cid. The N terminus of Cenp-C is further out, clustered with Mis12 and the Spc25 end of the rod-like Ndc80 complex, which is known to bind to microtubules at its other more distal Ndc80/Nuf2 end.
DOI	10.1007/s00412-007-0103-y
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Secondary IDs	FBrf0193710
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Chromosoma
Title	Chromosoma
Publication Year	1939-
ISBN/ISSN	0009-5915
Data from Reference
Aberrations (2)
	Df(2L)ade3 Df(3R)ry75
Alleles (31)
	Avic\GFP^{Jupiter-G00147} Bub3^{T:Avic\GFP-EGFP} Cenp-C^{C.T:Avic\GFP-EGFP} Cenp-C^{N.T:Avic\GFP-EGFP.C.T:Disc\RFP-mRFP} Cenp-C^{N.T:Avic\GFP-EGFP} Cenp-C^{N.T:Disc\RFP-mRFP.C.T:Avic\GFP-EGFP} Cenp-C^{T:Avic\GFP-EGFP} Cenp-C^{T:Avic\GFP-EYFP} cid^{T:Avic\GFP-EGFP} cid^{T:Disc\RFP-mRFP} Kmn1^{Scer\UAS.T:Avic\GFP-EGFP} Kmn2^{Scer\UAS.T:Avic\GFP-EGFP} Mis12^f03756 Mis12^{Scer\UAS.T:Avic\GFP-EGFP} Mis12^{T:Avic\GFP-EGFP} Ndc80^{Scer\UAS.T:Avic\GFP-EGFP} Ndc80^{T:Avic\GFP-EGFP} Nnf1a^{Scer\UAS.T:Avic\GFP-EGFP} Nnf1b^{Scer\UAS.T:Avic\GFP-EGFP} Nuf2^{Scer\UAS.T:Avic\GFP-EGFP} Nuf2^SH2276 Nuf2^{T:Avic\GFP-EGFP} Scer\GAL4^Sry-α.PS Spc25^A34-1 Spc25^c00064 Spc25^{Scer\UAS.T:Avic\GFP-EGFP} Spc25^{T:Avic\GFP-EGFP} Spc25^{T:Disc\RFP-mRFP} T:Avic\GFP^EGFP T:Disc\RFP^mRFP w^+mC
Constructs (15)
	P{Cenp-C.C.EGFP} P{Cenp-C.N.EGFP.C.RFP} P{Cenp-C.N.EGFP} P{Cenp-C.N.RFP.C.EGFP} P{gCenp-C-EGFP} P{gcid-EGFP-cid} P{gcid-mRFP-cid} P{gEGFP-Bub3} P{gEGFP-Ndc80} P{gEGFP-Nuf2} P{gEYFP-Cenp-C} P{gMis12-EGFP} P{gSpc25-EGFP} P{gSpc25-mRFP} P{Sry-α-GAL4.S}
Genes (26)
	Avic\GFP Bub3 Cenp-C cid Dana\Nnf1 Dere\Nnf1a Dere\Nnf1b Dper\Nnf1 Dpse\Nnf1 Dsec\Nnf1a Dsec\Nnf1b Dsim\Nnf1a Dsim\Nnf1b Dwil\Nnf1 Dyak\Nnf1a Dyak\Nnf1b Kmn1 Kmn2 Mis12 Ndc80 Nnf1a Nnf1b Nuf2 Scer\GAL4 Spc25 w
Insertions (10)
	P{gCenp-C-EGFP}III.2 P{gcid-EGFP-cid}III.2 P{gcid-mRFP-cid}II.1 P{gEGFP-Nuf2}II.1 P{gEYFP-Cenp-C}II.1 P{gMis12-EGFP}II.1 P{gSpc25-EGFP}II.1 P{gSpc25-mRFP}II.1 P{PTT-GA}Jupiter^G00147 P{Sry-α-GAL4.S}II.1
Natural transposons (1)
	P-element
Polypeptides (11)
	Bub3^{T:Avic\GFP-EGFP}PA cid^{T:Disc\RFP-mRFP}PA Kmn1^{Scer\UAS.T:Avic\GFP-EGFP}PA Kmn2^{Scer\UAS.T:Avic\GFP-EGFP}PA Mis12^{Scer\UAS.T:Avic\GFP-EGFP}PA Mis12^{T:Avic\GFP-EGFP}PA Ndc80^{T:Avic\GFP-EGFP}PA Nnf1a^{Scer\UAS.T:Avic\GFP-EGFP}PA Nnf1b^{Scer\UAS.T:Avic\GFP-EGFP}PA Nuf2^{T:Avic\GFP-EGFP}PA Spc25^{T:Avic\GFP-EGFP}PA