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| Citation | Bischof, J., Basler, K. (2007.11.7). Information on M{vas-int.Dm} stocks. (Export to RIS) | ||
| FlyBase ID | FBrf0200341 | ||
| Publication Type | Personal communication to FlyBase | ||
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| Text of Personal Communication |
To: flybase-updates@XXXXXXXXXXXXXXX
From: Kevin Cook <kcook@XXXXXXXXXXXXXXX> Subject: phiC31 constructs and insertions Date: Wed, 07 Nov 2007 10:46:12 -0500 The following information accompanied stocks donated to the Bloomington Stock Center by Johannes Bischof and Konrad Basler, University of Zürich. M{vas-int.Dm} carries a phiC31 integrase gene that has been engineered to have the codon bias of Drosophila melanogaster. Expression of the integrase is driven by the vasa promoter. The construction of M{vas-int.Dm} is similar to the construction of M{vas-int.B} (FBtp0022219), which was described in Bischof et al., 2007 (PNAS 104: 3312--3317; http://flybase.net/reports/FBrf0199320.html). Basically, the vasa promoter-phiC31 integrase sequence, marked with Avic\GFP[E.3xP3] (FBal0103092) and flanked by attB sites, was integrated into M{3xP3-RFP.attP} via phiC31 integrase-mediated recombination. Other than the altered integrase coding region, M{vas-int.Dm} is identical to M{vas-int.B}. The presence both of Avic\GFP[E.3xP3] and Disc\RFP[3xP3.PB] (FBal0193772) in M{vas-int.Dm} causes RFP and EGFP expression in late pupal and adult eyes. In practice, this leads to a pink eye color from the RFP gradually appearing in adult flies as they get older. This pink, however, is clearly distinct from the color resulting from expression of white. M{vas-int.Dm}ZH-2A is a homozygous and hemizygous viable and fertile, X chromosome insertion at 2A. It was isolated by phiC31 integrase-mediated recombination of the attB-vasa promoter-phiC31 integrase-attB sequence into M{3xP3-RFP.attP}ZH-2A. M{vas-int.Dm}ZH-102D is a homozygous viable and fertile, fourth chromosome insertion at 102D. It was isolated by phiC31 integrase-mediated recombination of the the attB-vasa promoter-phiC31 integrase-attB sequence into M{3xP3-RFP.attP}ZH-102D. In Bischof et al, 2007, the following insertions from Supporting Information Table 4 are insertions of M{3xP3-RFP.attP}: M{3xP3-RFP.attP}ZH-2A M{3xP3-RFP.attP}ZH-51D M{3xP3-RFP.attP}ZH-86Fb M{3xP3-RFP.attP}ZH-102D The following lines are insertions of M{3xP3-RFP.attP'}: M{3xP3-RFP.attP'}ZH-22A M{3xP3-RFP.attP'}ZH-51C M{3xP3-RFP.attP'}ZH-58A M{3xP3-RFP.attP'}ZH-68E M{3xP3-RFP.attP'}ZH-86Fa M{3xP3-RFP.attP'}ZH-96E M{3xP3-RFP.attP} and M{3xP3-RFP.attP'} differ by only a few nucleotides. |
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| Language of Publication | English | ||
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Data from Reference
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| Alleles (3)
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| Constructs (4)
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| Genes (3)
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| Insertions (12)
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| Natural transposons (1)
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