Open Close
Reference
Citation
Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck, J., Owen, S., Skora, A.D., Nystul, T.G., Ohlstein, B., Allen, A., Wilhelm, J.E., Murphy, T.D., Levis, R.W., Matunis, E., Srivali, N., Hoskins, R.A., Spradling, A.C. (2007). The Carnegie protein trap library: A versatile tool for Drosophila developmental studies.  Genetics 175(3): 1505--1531.
FlyBase ID
FBrf0200345
Publication Type
Research paper
Abstract
Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell resolution. By sequencing the genomic insertion sites, determining splicing patterns downstream of the enhanced green fluorescent protein (EGFP) exon, and analyzing expression patterns in the ovary and salivary gland, we found that 600-900 different genes are trapped in our collection. A core set of 244 lines trapped different identifiable protein isoforms, while insertions likely to act as GFP-enhancer traps were found in 256 additional genes. At least 8 novel genes were also identified. Our results demonstrate that the Carnegie collection will be useful as a discovery tool in diverse areas of cell and developmental biology and suggest new strategies for greatly increasing the coverage of the Drosophila proteome with protein trap insertions.
PubMed ID
PubMed Central ID
PMC1840051 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
FlyTrap insertion lines - genomic sequence locations.
Levis et al., 2013-, FlyTrap insertion lines - genomic sequence locations. [FBrf0222141]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0192944
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Genetics
Title
Genetics
Publication Year
1916-
ISBN/ISSN
0016-6731
Data From Reference
Alleles (940)
List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
Genes (505)
Datasets (2)
Natural transposons (2)
Insertions (517)
List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
Experimental Tools (2)
Transgenic Constructs (7)