Reference Report

Reference
Citation	Buszczak, M., Paterno, S., Lighthouse, D., Bachman, J., Planck, J., Owen, S., Skora, A.D., Nystul, T.G., Ohlstein, B., Allen, A., Wilhelm, J.E., Murphy, T.D., Levis, R.W., Matunis, E., Srivali, N., Hoskins, R.A., Spradling, A.C. (2007). The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. Genetics 175(3): 1505--1531. (Export to RIS)
FlyBase ID	FBrf0200345
Publication Type	Research paper
PubMed ID	17194782
PubMed Abstract	Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library of 7404 protein trap and enhancer trap lines, the Carnegie collection, to facilitate gene expression mapping at single-cell resolution. By sequencing the genomic insertion sites, determining splicing patterns downstream of the enhanced green fluorescent protein (EGFP) exon, and analyzing expression patterns in the ovary and salivary gland, we found that 600-900 different genes are trapped in our collection. A core set of 244 lines trapped different identifiable protein isoforms, while insertions likely to act as GFP-enhancer traps were found in 256 additional genes. At least 8 novel genes were also identified. Our results demonstrate that the Carnegie collection will be useful as a discovery tool in diverse areas of cell and developmental biology and suggest new strategies for greatly increasing the coverage of the Drosophila proteome with protein trap insertions.
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Secondary IDs	FBrf0192944
Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (945)
	All Alleles relating to this reference
Constructs (7)
	P{hsp70-pBACtrans.B} P{PTT-GA} P{PTT-GB} P{PTT-GC} P{Ubi-pBACtrans.B} PBac{BglII-GFP.A} PBac{HpaI-GFP.A}
Genes (506)
	All Genes relating to this reference
Insertions (517)
	All Insertions relating to this reference
Natural transposons (2)
	P-element Tni\piggyBac