Reference
Citation
Quinones-Coello, A.T., Petrella, L.N., Ayers, K., Melillo, A., Mazzalupo, S., Hudson, A.M., Wang, S., Castiblanco, C., Buszczak, M., Hoskins, R.A., Cooley, L. (2007). Exploring strategies for protein trapping in Drosophila.  Genetics 175(3): 1089--1104.
FlyBase ID
FBrf0200349
Publication Type
Research paper
Abstract
The use of fluorescent protein tags has had a huge impact on cell biological studies in virtually every experimental system. Incorporation of coding sequence for fluorescent proteins such as green fluorescent protein (GFP) into genes at their endogenous chromosomal position is especially useful for generating GFP-fusion proteins that provide accurate cellular and subcellular expression data. We tested modifications of a transposon-based protein trap screening procedure in Drosophila to optimize the rate of recovering useful protein traps and their analysis. Transposons carrying the GFP-coding sequence flanked by splice acceptor and donor sequences were mobilized, and new insertions that resulted in production of GFP were captured using an automated embryo sorter. Individual stocks were established, GFP expression was analyzed during oogenesis, and insertion sites were determined by sequencing genomic DNA flanking the insertions. The resulting collection includes lines with protein traps in which GFP was spliced into mRNAs and embedded within endogenous proteins or enhancer traps in which GFP expression depended on splicing into transposon-derived RNA. We report a total of 335 genes associated with protein or enhancer traps and a web-accessible database for viewing molecular information and expression data for these genes.
PubMed ID
PubMed Central ID
PMC1840052 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
FlyTrap insertion lines - genomic sequence locations.
Levis et al., 2013-, FlyTrap insertion lines - genomic sequence locations. [FBrf0222141]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0193052
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Genetics
Title
Genetics
Publication Year
1916-
ISBN/ISSN
0016-6731
Data From Reference
Alleles (45)
Genes (16)
Datasets (2)
Natural transposons (2)
Insertions (26)
Transgenic Constructs (6)