Reference Report

Reference
Citation	Quinones-Coello, A.T., Petrella, L.N., Ayers, K., Melillo, A., Mazzalupo, S., Hudson, A.M., Wang, S., Castiblanco, C., Buszczak, M., Hoskins, R.A., Cooley, L. (2007). Exploring strategies for protein trapping in Drosophila. Genetics 175(3): 1089--1104. (Export to RIS)
FlyBase ID	FBrf0200349
Publication Type	Research paper
PubMed ID	17179094
PubMed Abstract	The use of fluorescent protein tags has had a huge impact on cell biological studies in virtually every experimental system. Incorporation of coding sequence for fluorescent proteins such as green fluorescent protein (GFP) into genes at their endogenous chromosomal position is especially useful for generating GFP-fusion proteins that provide accurate cellular and subcellular expression data. We tested modifications of a transposon-based protein trap screening procedure in Drosophila to optimize the rate of recovering useful protein traps and their analysis. Transposons carrying the GFP-coding sequence flanked by splice acceptor and donor sequences were mobilized, and new insertions that resulted in production of GFP were captured using an automated embryo sorter. Individual stocks were established, GFP expression was analyzed during oogenesis, and insertion sites were determined by sequencing genomic DNA flanking the insertions. The resulting collection includes lines with protein traps in which GFP was spliced into mRNAs and embedded within endogenous proteins or enhancer traps in which GFP expression depended on splicing into transposon-derived RNA. We report a total of 335 genes associated with protein or enhancer traps and a web-accessible database for viewing molecular information and expression data for these genes.
DOI
Related Publication(s)
Supplementary material	Supplemental Table 2. Lethality complementation tests of protein trap lines with Deficiency stocks. [FBrf0200389] Supplemental Table 1. List of genes associated with protein or enhancer traps. [FBrf0200393]
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Secondary IDs	FBrf0193052
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Alleles (49)
	Avic\GFP^Cam-P00695 Avic\GFP^{CG42258-G00035} Avic\GFP^Fas3-YD0853 Avic\GFP^{Fas3-ZCL02154} Avic\GFP^{GA.T:Zzzz\His6} Avic\GFP^{GB.T:Zzzz\His6} Avic\GFP^{GC.T:Zzzz\His6} Avic\GFP^Imp-G00080 Avic\GFP^Imp-G00152 Avic\GFP^Imp-YB0057 Avic\GFP^Imp-YD0166 Avic\GFP^Indy-YC0017 Avic\GFP^Oda-G00273 Avic\GFP^Oda-YD0523 Avic\GFP^Pdi-YD0170 Avic\GFP^Pdi-ZCL01514 Avic\GFP^Rtnl1-G00071 Avic\GFP^sgg-G00015 Avic\GFP^sgg-YD0060 Avic\GFP^tral-G01240 Avic\GFP^YB0011 Avic\GFP^YB0147 Avic\GFP^YB0172 Avic\GFP^YD0184 Avic\GFP^YD0570 Avic\GFP^{Zasp66-ZCL0663} Avic\GFP^ZCL2825 Avic\GFP^ZCL2860 Avic\GFP^ZCL3170 Cam^P00695 CG42258^G00035 Fas3^YD0853 Fas3^ZCL02154 Imp^G00080 Imp^G00152 Imp^YB0057 Imp^YD0166 Indy^YC0017 Oda^G00273 Oda^YD0523 Pdi^YD0170 Pdi^ZCL01514 Rtnl1^G00071 sgg^G00015 sgg^YD0060 Tni\piggyBac\T^Ubi.PB tral^G01240 y^+mDint2 Zasp66^ZCL0663
Constructs (6)
	P{PTT-GA} P{PTT-GB} P{PTT-GC} P{PTT-un1} P{Ubi-pBACtrans.B} PBac{HpaI-GFP.A}
Genes (16)
	Avic\GFP Cam CG42258 D1 Fas3 Imp Indy Oda Pdi RpS2 Rtnl1 sgg Tni\piggyBac\T tral y Zasp66
Insertions (26)
	P{PTT-GA}Zasp66^ZCL0663 P{PTT-GB}Imp^YB0057 P{PTT-GB}YB0011 P{PTT-GB}YB0147 P{PTT-GB}YB0172 P{PTT-GC}Indy^YC0017 P{PTT-un1}Cam^P00695 P{PTT-un1}CG42258^G00035 P{PTT-un1}Fas3^ZCL02154 P{PTT-un1}Imp^G00080 P{PTT-un1}Imp^G00152 P{PTT-un1}Oda^G00273 P{PTT-un1}Pdi^ZCL01514 P{PTT-un1}Rtnl1^G00071 P{PTT-un1}sgg^G00015 P{PTT-un1}tral^G01240 P{PTT-un1}ZCL2825 P{PTT-un1}ZCL2860 P{PTT-un1}ZCL3170 PBac{HpaI-GFP.A}Fas3^YD0853 PBac{HpaI-GFP.A}Imp^YD0166 PBac{HpaI-GFP.A}Oda^YD0523 PBac{HpaI-GFP.A}Pdi^YD0170 PBac{HpaI-GFP.A}sgg^YD0060 PBac{HpaI-GFP.A}YD0184 PBac{HpaI-GFP.A}YD0570
Natural transposons (2)
	P-element Tni\piggyBac