Open Close
Reference
Citation
Ni, J.Q., Markstein, M., Binari, R., Pfeiffer, B., Liu, L.P., Villalta, C., Booker, M., Perkins, L., Perrimon, N. (2008). Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster.  Nat. Methods 5(1): 49--51.
FlyBase ID
FBrf0200406
Publication Type
Research paper
Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
PubMed ID
PubMed Central ID
PMC2290002 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
VALIUM1 lines added to the TRiP stock collection.
Perkins et al., 2010.7.26, VALIUM1 lines added to the TRiP stock collection. [FBrf0211374]
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Methods
    Title
    Nature Methods
    Publication Year
    2004-
    ISBN/ISSN
    1548-7091 1548-7105
    Data From Reference
    Alleles (681)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (540)
    Datasets (1)
    Molecular Constructs (1)
    Sequence Features (678)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Natural transposons (1)
    Insertions (681)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (3)
    Transgenic Constructs (681)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.