Reference Report

Reference
Citation	Ni, J.Q., Markstein, M., Binari, R., Pfeiffer, B., Liu, L.P., Villalta, C., Booker, M., Perkins, L., Perrimon, N. (2008). Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nat. Methods 5(1): 49--51. (Export to RIS)
FlyBase ID	FBrf0200406
Publication Type	Research paper
PubMed ID	18084299
PubMed Abstract	The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site-driven RNA interference constructs have been inserted into the genome randomly using P-element-mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
DOI	10.1038/nmeth1146
Related Publication(s)
Research paper	A Drosophila Resource of Transgenic RNAi Lines for Neurogenetics. Ni et al., 2009, Genetics 182(4): 1089--1100 [FBrf0208670]
Personal communication to FlyBase	Initial TRiP stock collection. Perkins et al., 1.15.2009, Initial TRiP stock collection. [FBrf0206489] Update to TRiP stock collection. Perkins et al., 2009.8.10, Update to TRiP stock collection. [FBrf0208863] Update to TRiP stock collection. Perkins et al., 2009, Update to TRiP stock collection. [FBrf0208864] A genome-scale shRNA resource for transgenic RNAi in Drosophila. Ni et al., 2010.12.1, A genome-scale shRNA resource for transgenic RNAi in Drosophila. [FBrf0212437] TRiP germline vector pVALIUM21. Ni et al., 2011.7.19, TRiP germline vector pVALIUM21. [FBrf0214641]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Methods
Title	Nature Methods
Publication Year	2004-
ISBN/ISSN	1548-7091 1548-7105
Data from Reference
Alleles (681)
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Constructs (682)
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Genes (541)
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Insertions (681)
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Libraries/Collections (1)
	TRiP-2
Sequence features (678)
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