Open Close
Reference
Citation
Srivastava, M., Scherr, H., Lackey, M., Xu, D., Chen, Z., Lu, J., Bergmann, A. (2007). ARK, the Apaf-1 related killer in Drosophila, requires diverse domains for its apoptotic activity.  Cell Death Differ. 14(1): 92--102.
FlyBase ID
FBrf0200411
Publication Type
Research paper
Abstract

In mammals and Drosophila, apoptotic caspases are under positive control of the CED-4-like proteins Apaf-1 and ARK, respectively. In an EMS-mutagenesis screen, we isolated 33 ark mutants as recessive suppressors of hid-induced apoptosis. The ark mutants are loss-of-function alleles characterized by reduced developmental apoptosis. Using the phenotypic series of these alleles, we identified helical domain I in the nucleotide oligomerization domain as critical for ARK's apoptotic activity. Interestingly, the WD40 region may also have an unanticipated positive requirement for the apoptotic activity of ARK. Considering structural information, we discuss the roles of these domains for assembly and activity of the ARK apoptosome, and propose that the WD40 region is anti-apoptotic in the absence of apoptotic signals, and pro-apoptotic in the presence of such signals. Furthermore, a defined null allele reveals that ark is required for most, but not all apoptosis suggesting the existence of an ARK-independent apoptotic pathway.

PubMed ID
PubMed Central ID
PMC2502064 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
  • FBrf0189951
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
Cell Death Differ.
Title
Cell Death and Differentiation
Publication Year
1994-
ISBN/ISSN
1350-9047
Data From Reference