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Citation
Kind, J., Akhtar, A. (2007). Cotranscriptional recruitment of the dosage compensation complex to X-linked target genes.  Genes Dev. 21(16): 2030--2040.
FlyBase ID
FBrf0200424
Publication Type
Research paper
Abstract
Dosage compensation is a process required to balance the expression of X-linked genes between males and females. In Drosophila this is achieved by targeting the dosage compensation complex or the male-specific lethal (MSL) complex to the male X chromosome. In order to study the mechanism of targeting, we have studied two X-chromosomal genes, mof and CG3016, using chromatin immunoprecipitation as well as immuno-FISH analysis on transgenic flies. We show that MSL complex recruitment requires the genes to be in a transcriptionally active state. MSL complex recruitment is reversible because blocking transcription severely reduces MSL binding to its target genes. Furthermore, targeting cues are found toward the 3' end of the gene and depend on the passage of the transcription machinery through the gene, whereby the type of promoter and the direction of transcription are dispensable. We propose a model of dynamic MSL complex binding to active genes based on exposed DNA target elements.
PubMed ID
PubMed Central ID
PMC1948858 (PMC) (EuropePMC)
Related Publication(s)
Note
X chromosome exposé.
Lall, 2007, Nat. Struct. Mol. Biol. 14(9): 794 [FBrf0216084]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference