Reference Report

Reference
Citation	Song, Y., He, F., Xie, G., Guo, X., Xu, Y., Chen, Y., Liang, X., Stagljar, I., Egli, D., Ma, J., Jiao, R. (2007). CAF-1 is essential for Drosophila development and involved in the maintenance of epigenetic memory. Dev. Biol. 311(1): 213--222. (Export to RIS)
FlyBase ID	FBrf0200457
Publication Type	Research paper
PubMed ID	17916346
PubMed Abstract	DNA synthesis during S-phase and upon DNA repair is accompanied by chromatin assembly. The chromatin assembly factor CAF-1 has been biochemically well-characterized to deposit histones onto newly synthesized DNA. To gain insights into the in vivo functions of CAF-1 in Drosophila, we generated null mutants of the largest subunit of dCAF-1, dCAF-1-p180. We show that, unlike CAF-1 mutant yeast, dCAF-1-p180 mutant flies are hemizygous lethal. Removal of maternal dCAF-1-p180 activity by germline clones blocks oogenesis. Tissue-specific deletion of dCAF-1-p180 in the eye primordia disrupts eye development. In addition, reduction of dCAF-1-p180 activity suppresses gene silencing at heterochromatin and antagonizes Polycomb-mediated cell fate determination. Furthermore, heterozygous dCAF-1-p180 mutant flies display an increased sensitivity to gamma-irradiation and a reduced efficiency in recombinational double strand break (DSB) repair. Our experiments also show that human hCAF-1-p150 can rescue the dCAF-1-p180 mutant flies, demonstrating a functional conservation of eukaryotic CAF-1 activities in vivo. Together, our results establish that dCAF-1-p180 is an essential gene for Drosophila development and further underscore the importance of dCAF-1 in regulating gene expression and DNA repair in vivo.
DOI	10.1016/j.ydbio.2007.08.039
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Aberrations (3)
	Df(1)JA27 In(2)Heidi T(2;3)Sb^V
Alleles (24)
	asf1¹ asf1² asf1^Scer\UAS.cMa BacA\p35^Scer\UAS.cHa Blm^D2 Blm^Scer\UAS.cSa Caf1-180¹⁰⁰ Caf1-180²⁷⁰ Caf1-180³¹⁴ Caf1-180^Scer\FRT Caf1-180^Scer\UAS.cSa Caf1-180^{Scer\UAS.T:Disc\RFP-DsRed2} Caf1-180^unspecified His3^{hs.T:Avic\GFP} Hsap\CHAF1A^Scer\UAS.cSa Pc³ Scer\FLP1^ey.PN Scer\GAL4^Act.PU Scer\GAL4^ey.PH Scer\GAL4^tub.PU Scer\GAL4^unspecified T:Disc\RFP^DsRed2 y¹ y^F.TARG
Balancers (1)
	In(2)Heidi-P{Winkelried}D
Constructs (15)
	P{(-FRT).S} P{Act-GAL4.U} P{ey-FLP.N} P{FRT(Caf1-180).S} P{GAL4} P{GAL4-ey.H} P{hs-His3.GFP} P{tub-GAL4.U} P{UAS-asf1.M} P{UAS-Caf1-180.dsRed} P{UAS-Caf1-180.S} P{UAS-hCAF-1-p150} P{UAS-mus309.cSa} P{UAS-p35.H} P{y.F.TARG}
Genes (15)
	asf1 BacA\p35 Blm Caf1-180 dlg1 His3 Hsap\CHAF1A Mbs Pc rok Scer\FLP1 Scer\GAL4 shg sqh y
Insertions (3)
	P{GT1}BG02681 P{Winkelried}D P{y^tF-R}5
Natural transposons (1)
	P-element