Reference Report

Reference
Citation	White, A.E., Leslie, M.E., Calvi, B.R., Marzluff, W.F., Duronio, R.J. (2007). Developmental and cell cycle regulation of the Drosophila histone locus body. Mol. Biol. Cell 18(7): 2491--2502. (Export to RIS)
FlyBase ID	FBrf0200471
Publication Type	Research paper
PubMed ID	17442888
PubMed Abstract	Cyclin E/Cdk2 is necessary for replication-dependent histone mRNA biosynthesis, but how it controls this process in early development is unknown. We show that in Drosophila embryos the MPM-2 monoclonal antibody, raised against a phosphoepitope from human mitotic cells, detects Cyclin E/Cdk2-dependent nuclear foci that colocalize with nascent histone transcripts. These foci are coincident with the histone locus body (HLB), a Cajal body-like nuclear structure associated with the histone locus and enriched in histone pre-mRNA processing factors such as Lsm11, a core component of the U7 small nuclear ribonucleoprotein. Using MPM-2 and anti-Lsm11 antibodies, we demonstrate that the HLB is absent in the early embryo and occurs when zygotic histone transcription begins during nuclear cycle 11. Whereas the HLB is found in all cells after its formation, MPM-2 labels the HLB only in cells with active Cyclin E/Cdk2. MPM-2 and Lsm11 foci are present in embryos lacking the histone locus, and MPM-2 foci are present in U7 mutants, which cannot correctly process histone pre-mRNA. These data indicate that MPM-2 recognizes a Cdk2-regulated protein that assembles into the HLB independently of histone mRNA biosynthesis. HLB foci are present in histone deletion embryos, although the MPM-2 foci are smaller, and some Lsm11 foci are not associated with MPM-2 foci, suggesting that the histone locus is important for HLB integrity.
DOI	10.1091/mbc.E06-11-1033
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Aberrations (2)
	Df(2L)DS6 Df(3R)stg^AR2
Alleles (6)
	CycE^AR95 CycE^hs.PR Lsm11^{Scer\UAS.N.T:Avic\GFP-YFP.Venus} Slbp¹⁵ snRNA:U7¹⁴ stg⁴
Constructs (2)
	P{hsp70-DmcycE} P{UASp-YFP-Lsm11}
Genes (6)
	CycE His3 Lsm11 Slbp snRNA:U7 stg