Reference Report

Reference
Citation	Rathke, C., Baarends, W.M., Jayaramaiah-Raja, S., Bartkuhn, M., Renkawitz, R., Renkawitz-Pohl, R. (2007). Transition from a nucleosome-based to a protamine-based chromatin configuration during spermiogenesis in Drosophila. J. Cell Sci. 120(9): 1689--1700. (Export to RIS)
FlyBase ID	FBrf0200503
Publication Type	Research paper
PubMed ID	17452629
PubMed Abstract	In higher organisms, the chromatin of sperm is organised in a highly condensed protamine-based structure. In pre-meiotic stages and shortly after meiosis, histones carry multiple modifications. Here, we focus on post-meiotic stages and show that also after meiosis, histone H3 shows a high overall methylation of K9 and K27 and we hypothesise that these modifications ensure maintenance of transcriptional silencing in the haploid genome. Furthermore, we show that histones are lost during the early canoe stage and that just before this stage, hyper-acetylation of histone H4 and mono-ubiquitylation of histone H2A occurs. We believe that these histone modifications within the histone-based chromatin architecture may lead to better access of enzymes and chromatin remodellers. This notion is supported by the presence of the architectural protein CTCF, numerous DNA breaks, SUMO, UbcD6 and high content of ubiquitin, as well as testes-specific nuclear proteasomes at this time. Moreover, we report the first transition protein-like chromosomal protein, Tpl(94D), to be found in Drosophila. We propose that Tpl(94D)--an HMG box protein--and the numerous DNA breaks facilitate chromatin unwinding as a prelude to protamine and Mst77F deposition. Finally, we show that histone modifications and removal are independent of protamine synthesis.
DOI	10.1242/jcs.004663
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Language of Publication	English
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Publication Type	Journal
Abbreviation	J. Cell Sci.
Title	Journal of Cell Science
Publication Year	1966-
ISBN/ISSN	0021-9533
Data from Reference
Aberrations (2)
	Df(2L)protΔ38.1 Df(3L)ri-79c
Alleles (35)
	bel^cap-1 bet¹ blw¹ Bruce^E81 Bsg^gel cbx⁰⁵⁷⁰⁴ Cyt-c-d^bln1 dolly-1⁰³⁸¹⁷ eff⁸ emm¹ esc¹ gish⁰⁴⁸⁹⁵ gor¹ goul¹ His2Av^T:Disc\RFP His3.3A^T:Zzzz\FLAG His3^{His3.3A.T:Zzzz\FLAG} mlt¹ mod⁰⁷⁵⁷⁰ ms(2)29F⁰⁷⁷¹⁷ ms(2)42D⁰⁹⁹⁶⁷ ms(3)72D⁰³⁹⁵⁷ ms(3)85D⁰³⁵⁶⁵ ms(3)98B⁰⁶³⁰² Mst35Bb^{T:Avic\GFP-EGFP} Mst77F¹ pea¹ poe⁰¹⁶⁵⁹ Prosα3^{T:Avic\GFP-EGFP} Prosα3T^{T:Avic\GFP-EGFP} sdl^05090a T:Avic\GFP^EGFP tho¹ tmx¹ Tpl94D^{T:Avic\GFP-EGFP}
Constructs (7)
	P{His2Av-RFP.W} P{His3.3A-Flag} P{His3-Flag} P{Prosα3-EGFP.M} P{Prosα3T-EGFP.M} P{protamineB-eGFP} P{Tpl94D-eGFP.R}
Genes (52)
	bel bet blw Bruce Bsg cbx CG4480 CG4691 CG15278 CG15279 CG15510 CG31861 CG33309 CG42355 CTCF Cyt-c-d dj djl dolly-1 eff emm esc gish gor goul His2A His2Av His3 His3.3A His4 mlt mod ms(2)29F ms(2)42D ms(3)72D ms(3)85D ms(3)98B Mst35Ba Mst35Bb Mst77F pea poe Prosα3 Prosα3T RpII215 sdl smt3 T:Disc\RFP tho tmx Tpl94D UbcD6
Natural transposons (1)
	P-element