Reference Report

Reference
Citation	Förstemann, K., Horwich, M.D., Wee, L., Tomari, Y., Zamore, P.D. (2007). Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by Dicer-1. Cell 130(2): 287--297. (Export to RIS)
FlyBase ID	FBrf0200521
Publication Type	Research paper
PubMed ID	17662943
PubMed Abstract	Small interfering RNAs (siRNAs) and microRNAs (miRNAs) guide distinct classes of RNA-induced silencing complexes (RISCs) to repress mRNA expression in biological processes ranging from development to antiviral defense. In Drosophila, separate but conceptually similar endonucleolytic pathways produce siRNAs and miRNAs. Here, we show that despite their distinct biogenesis, double-stranded miRNAs and siRNAs participate in a common sorting step that partitions them into Ago1- or Ago2-containing effector complexes. These distinct complexes silence their target RNAs by different mechanisms. miRNA-loaded Ago2-RISC mediates RNAi, but only Ago1 is able to repress an mRNA with central mismatches in its miRNA-binding sites. Conversely, Ago1 cannot mediate RNAi, because it is an inefficient nuclease whose catalytic rate is limited by the dissociation of its reaction products. Thus, the two members of the Drosophila Ago subclade of Argonaute proteins are functionally specialized, but specific small RNA classes are not restricted to associate with Ago1 or Ago2.
DOI	10.1016/j.cell.2007.05.056
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	Cell
Title	Cell
Publication Year	1974-
ISBN/ISSN	0092-8674
Data from Reference
Alleles (14)
	AGO1^dsRNA.cFa AGO2^dsRNA.cFa Avic\GFP^2xmir-277 Avic\GFP^{4xmir-277.bulged} Avic\GFP^dsRNA Dcr-1^dsRNA.cFa Dcr-2^dsRNA.cFa Dcr-2^G31R Dcr-2^L811fsX drosha^dsRNA.cFa loqs^dsRNA.cFa mir-277^pri.Ubi r2d2¹ r2d2^dsRNA.cFb
Constructs (2)
	P{GFP-2xmir-277} P{GFP-4xmir-277-bulged}
Genes (11)
	AGO1 AGO2 Avic\GFP ban Dcr-1 Dcr-2 drosha let-7 loqs mir-277 r2d2
Natural transposons (1)
	P-element