Reference Report

Reference
Citation	Mondal, K., Dastidar, A.G., Singh, G., Madhusudhanan, S., Gande, S.L., VijayRaghavan, K., Varadarajan, R. (2007). Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila. J. Mol. Biol. 370(5): 939--950. (Export to RIS)
FlyBase ID	FBrf0200583
Publication Type	Research paper
PubMed ID	17553522
PubMed Abstract	Little is known about mechanisms responsible for the temperature-sensitive (ts) phenotype, or of the transferability of ts mutants of a specific gene between organisms. Using a structure-based approach, nine ts mutants of Gal4 were generated in yeast by mutating four DNA binding residues. Two of these nine yeast ts mutants were cloned into P element vectors under control of the Elav and GMR promoters and transgenic Drosophila lines were generated. These were crossed to UAS reporter lines and progeny were characterized for reporter gene expression as a function of temperature. Both of these yeast ts mutants show a ts phenotype in Drosophila and result in rapid induction of reporter gene expression upon shifting to the permissive temperature. Exposed, functional residues involved in protein-ligand or protein-protein interactions appear to be attractive candidate sites for generating ts mutants that are transferable between organisms.
DOI	10.1016/j.jmb.2007.05.035
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Secondary IDs	FBrf0205052
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Mol. Biol.
Title	Journal of Molecular Biology
Publication Year	1959-
ISBN/ISSN	0022-2836
Data from Reference
Alleles (12)
	Ctet\TeTxLC^TNT.Scer\UAS Ecol\lacZ^{Scer\UAS.T:SV40\nls2} Scer\GAL4^elav.PLu Scer\GAL4^GMR.PM Scer\GAL4^K23P.elav Scer\GAL4^K23P.GMR Scer\GAL4^R15W.elav Scer\GAL4^R15W.GMR Scer\GAL4^{R15W.K23P.elav} Scer\GAL4^{R15W.K23P.GMR} Scer\GAL80^αTub84B.PL W^Scer\UAS.cZa
Constructs (12)
	P{elav-GAL4.K23P} P{elav-GAL4.R15W.K23P} P{elav-GAL4.R15W} P{GAL4-elav.L} P{GMR-fGal4} P{GMR-GAL4.K23P} P{GMR-GAL4.R15W.K23P} P{GMR-GAL4.R15W} P{tubP-GAL80} P{UAS-lacZ.NZ} P{UAS-TeTxLC.tnt} P{UAS-W.Z}
Genes (7)
	Ctet\TeTxLC Ecol\lacZ elav GMR Scer\GAL4 Scer\GAL80 W
Natural transposons (1)
	P-element