Reference Report

Reference
Citation	Popichenko, D., Sellin, J., Bartkuhn, M., Paululat, A. (2007). Hand is a direct target of the forkhead transcription factor Biniou during Drosophila visceral mesoderm differentiation. BMC Dev. Biol. 7(): 49. (Export to RIS)
FlyBase ID	FBrf0200603
Publication Type	Research paper
PubMed ID	17511863
PubMed Abstract	The visceral trunk mesoderm in Drosophila melanogaster develops under inductive signals from the ectoderm. This leads to the activation of the key regulators Tinman, Bagpipe and Biniou that are crucial for specification of the circular visceral muscles. How further differentiation is regulated is widely unknown, therefore it seems to be essential to identify downstream target genes of the early key regulators. In our report we focus on the analysis of the transcriptional control of the highly conserved transcription factor Hand in circular visceral muscle cells, providing evidence that the hand gene is a direct target of Biniou.Herein we describe the identification of a regulatory region in the hand gene essential and sufficient for the expression in the visceral mesoderm during embryogenesis. We found that hand expression in the circular visceral mesoderm is abolished in embryos mutant for the FoxF domain containing transcription factor Biniou. Furthermore we demonstrate that Biniou regulates hand expression by direct binding to a 300 bp sequence element, located within the 3rd intron of the hand gene. This regulatory element is highly conserved in different Drosophila species. In addition, we provide evidence that Hand is dispensable for the initial differentiation of the embryonic visceral mesoderm.In the present report we show that cross species sequence comparison of non-coding sequences between orthologous genes is a powerful tool to identify conserved regulatory elements. Combining functional dissection experiments in vivo and protein/DNA binding studies we identified hand as a direct target of Biniou in the circular visceral muscles.
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Language of Publication	English
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Publication Type	Journal
Abbreviation	BMC Dev. Biol.
Title	BMC Developmental Biology
Publication Year	2002
ISBN/ISSN	1471-213X
Data from Reference
Aberrations (1)
	Df(2L)Exel7046
Alleles (14)
	Avic\GFP^{Hand.1054-1400} Avic\GFP^Hand.Bin1mut Avic\GFP^{Hand.Bin2mut4} Avic\GFP^Hand.Bin2mut Avic\GFP^Hand.Bin3mut Avic\GFP^Hand.Bin4mut Avic\GFP^{Hand.Δ395-824} Avic\GFP^{Hand.Δ1074-1374} Avic\GFP^HandC.1611 bin^I1 Ecol\lacZ^bap.3 h^unspecified Hand^ko lmd¹
Constructs (10)
	P{bap-lacZ.3} P{Hand.1611.EGFP.Stinger} P{Hand-GFP.1054-1400} P{Hand-GFP.Bin1mut} P{Hand-GFP.Bin2mut4} P{Hand-GFP.Bin2mut} P{Hand-GFP.Bin3mut} P{Hand-GFP.Bin4mut} P{Hand-GFP.Δ395-824} P{Hand-GFP.Δ1074-1374}
Genes (14)
	Alk Avic\GFP bap bin dpp Ecol\lacZ Fas3 h Hand jeb lmd sns tin βTub60D
Natural transposons (1)
	P-element
Sequence features (1)
	Hand_HV-element