Reference Report

Reference
Citation	Liu, R., Woolner, S., Johndrow, J.E., Metzger, D., Flores, A., Parkhurst, S.M. (2008). Sisyphus, the Drosophila myosin XV homolog, traffics within filopodia transporting key sensory and adhesion cargos. Development 135(1): 53--63. (Export to RIS)
FlyBase ID	FBrf0200619
Publication Type	Research paper
PubMed ID	18045836
PubMed Abstract	Unconventional myosin proteins of the MyTH-FERM superclass are involved in intrafilopodial trafficking, are thought to be mediators of membrane-cytoskeleton interactions, and are linked to several forms of deafness in mammals. Here we show that the Drosophila myosin XV homolog, Sisyphus, is expressed at high levels in leading edge cells and their cellular protrusions during the morphogenetic process of dorsal closure. Sisyphus is required for the correct alignment of cells on opposing sides of the fusing epithelial sheets, as well as for adhesion of the cells during the final zippering/fusion phase. We have identified several putative Sisyphus cargos, including DE-cadherin (also known as Shotgun) and the microtubule-linked proteins Katanin-60, EB1, Milton and aPKC. These cargos bind to the Sisyphus FERM domain, and their binding is in some cases mutually exclusive. Our data suggest a mechanism for Sisyphus in which it maintains a balance between actin and microtubule cytoskeleton components, thereby contributing to cytoskeletal cross-talk necessary for regulating filopodial dynamics during dorsal closure.
DOI	10.1242/dev.011437
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Alleles (10)
	Act5C^{Scer\UAS.P\T.T:Disc\RFP-mRFP} Act5C^{Scer\UAS.T:Avic\GFP} Avic\GFP^{Scer\UAS.T:Ecol\lacZ,T:SV40\nls2} Myo10A^{Scer\UAS.P\T.T:Avic\GFP} Myo10A^{Scer\UAS.P\T.T:Disc\RFP-mRFP} Scer\GAL4^en-e16E shg^{Scer\UAS.T:Avic\GFP-rs} T:Disc\RFP^mCherry T:Disc\RFP^mRFP αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (7)
	P{UAS-Act5C.T:GFP} P{UAS-GFP::lacZ.nls} P{UASp-Act5C.mRFP} P{UASp-GFPS65C-αTub84B} P{UASp-Myo10A.GFP} P{UASp-Myo10A.RFP} P{UAS-shg.DEFL}
Genes (33)
	Act5C aPKC Avic\GFP Bap60 CG2258 CG2765 CG5613 CG6834 CG18135 ck Cpsf160 crp Eb1 elgi Fip1 Hsp26 inc Kap-α3 Kat60 milt Myo10A Myo28B1 Nedd4 RpL23 RpL34a Scer\GAL4 shg smg Socs16D T:Avic\GFP tgo αTub84B αTub84D
Insertions (1)
	P{en2.4-GAL4}e16E