Reference Report

Reference
Citation	Yang, J., O'Tousa, J.E. (2007). Cellular sites of Drosophila NinaB and NinaD activity in vitamin a metabolism. Mol. Cell. Neurosci. 35(1): 49--56. (Export to RIS)
FlyBase ID	FBrf0200667
Publication Type	Research paper
PubMed ID	17344064
PubMed Abstract	The Drosophila genes ninaB and ninaD, encoding a beta-carotene oxygenase and a type B scavenger receptor respectively, are essential for the biosynthesis of the 3-hydroxyretinal chromophore of rhodopsin. We analyzed transgenic reporter strains and performed in situ hybridization to show that both ninaB and ninaD are expressed in the adult brain but not retinal tissues. Developmental RT-PCR and tissue expression studies showed that ninaB is only expressed in the adult brain, while ninaD is expressed in the adult brain, the adult body, and many larval tissues. The data support a model in which NinaD is required for uptake and storage of dietary carotenoids throughout the larval and adult stages of development. Beta-carotene is transported to the adult brain, where cellular uptake by NinaD allows cleavage by the NinaB enzyme to produce retinal. Retinal is then transported to the retina for rhodopsin biogenesis.
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Secondary IDs	FBrf0194027
Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Cell. Neurosci.
Title	Molecular and Cellular Neurosciences
Publication Year	1990-
ISBN/ISSN	1044-7431
Data from Reference
Alleles (9)
	Avic\GFP^E.Scer\UAS Avic\GFP^ninaD.PY ninaB¹ ninaB^Scer\UAS.cGa ninaD² ninaD^Scer\UAS.cKa ninaE^unspecified Scer\GAL4^ninaB.PY Scer\GAL4^Scer\UAS.cHa
Constructs (6)
	P{ninaB-GAL4.Y} P{ninaD-GFP.Y} P{UAS-EGFP} P{UAS-Gal4.H} P{UAS-ninaB.G} P{UAS-ninaD}
Genes (5)
	Avic\GFP ninaB ninaD ninaE Scer\GAL4
Natural transposons (1)
	P-element
Transcripts (2)
	Avic\GFP^ninaD.PYRA Scer\GAL4^ninaB.PYRA