Reference Report

Reference
Citation	Ferjoux, G., Auge, B., Boyer, K., Haenlin, M., Waltzer, L. (2007). A GATA/RUNX cis-regulatory module couples Drosophila blood cell commitment and differentiation into crystal cells. Dev. Biol. 305(2): 726--734. (Export to RIS)
FlyBase ID	FBrf0200672
Publication Type	Research paper
PubMed ID	17418114
PubMed Abstract	Members of the RUNX and GATA transcription factor families play critical roles during hematopoiesis from Drosophila to mammals. In Drosophila, the formation of the crystal cell hematopoietic lineage depends on the continuous expression of the lineage-specific RUNX factor Lozenge (Lz) and on its interaction with the GATA factor Serpent (Srp). Crystal cells are the main source of prophenoloxidases (proPOs), the enzymes required for melanization. By analyzing the promoter regions of several insect proPOs, we identify a conserved GATA/RUNX cis-regulatory module that ensures the crystal cell-specific expression of the three Drosophila melanogaster proPO. We demonstrate that activation of this module requires the direct binding of both Srp and Lz. Interestingly, a similar GATA/RUNX signature is over-represented in crystal cell differentiation markers, allowing us to identify new Srp/Lz target genes by genome-wide screening of Drosophila promoter regions. Finally, we show that the expression of lz in the crystal cells also relies on Srp/Lz-mediated activation via a similar module, indicating that crystal cell fate choice maintenance and activation of the differentiation program are coupled. Based on our observations, we propose that this GATA/RUNX cis-regulatory module may be reiteratively used during hematopoietic development through evolution.
DOI	10.1016/j.ydbio.2007.03.010
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (13)
	Ecol\lacZ^{lz.144.GATAmut} Ecol\lacZ^{lz.144.RUNXmut} Ecol\lacZ^lz.144 Ecol\lacZ^{proPO45.GATAmut} Ecol\lacZ^proPO45.PF Ecol\lacZ^{proPO45.RUNXmut} Ecol\lacZ^proPO59.PF Ecol\lacZ^proPO-A1.PF lz^r1 lz^Scer\UAS.cBa Scer\GAL4^lz-gal4 Scer\GAL4^twi.PG srp^NC.Scer\UAS
Constructs (11)
	P{GAL4-twi.G} P{lz-lacZ.144.GATAmut} P{lz-lacZ.144.RUNXmut} P{lz-lacz.144} P{proPO45-lacZ.F} P{proPO45-lacZ.GATAmut} P{proPO45-lacZ.RUNXmut} P{proPO59-lacZ.F} P{proPO-A1-lacZ.F} P{UAS-lz.B} P{UAS-srp.NC}
Genes (57)
	Ald BTBD9 BtbVII Cdk4 CG2970 CG3332 CG3744 CG5697 CG6475 CG6733 CG7328 CG7860 CG8944 CG9445 CG10602 CG10650 CG10725 CG11089 CG12493 CG13565 CG14183 CG14269 CG14823 CG16716 CG17224 CG18745 CG30421 CG31086 CG31102 CG32694 cv-c Dh44-R1 Dot dpr5 Ecol\lacZ Ets96B Irk1 Jon65Aiii Lapsyn lz Mlf Myo61F Nc73EF Nup205 Oscillin par-1 pico Pink1 proPO45 proPO59 proPO-A1 Reg-2 RhoGEF3 Scer\GAL4 srp Su(Tpl) α-Man-Ib
Insertions (1)
	P{GawB}lz^gal4
Natural transposons (1)
	P-element
Sequence features (2)
	CG42640_proPO59crystal_cell proPO-A1_crystal_cell