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Citation
Koizumi, K., Higashida, H., Yoo, S., Islam, M.S., Ivanov, A.I., Guo, V., Pozzi, P., Yu, S.H., Rovescalli, A.C., Tang, D., Nirenberg, M. (2007). RNA interference screen to identify genes required for Drosophila embryonic nervous system development.  Proc. Natl. Acad. Sci. U.S.A. 104(13): 5626--5631.
FlyBase ID
FBrf0200690
Publication Type
Research paper
Abstract
RNA interference (RNAi) has been shown to be a powerful method to study the function of genes in vivo by silencing endogenous mRNA with double-stranded (ds) RNA. Previously, we performed in vivo RNAi screening and identified 43 Drosophila genes, including 18 novel genes required for the development of the embryonic nervous system. In the present study, 22 additional genes affecting embryonic nervous system development were found. Novel RNAi-induced phenotypes affecting nervous system development were found for 16 of the 22 genes. Seven of the genes have unknown functions. Other genes found encode transcription factors, a chromatin-remodeling protein, membrane receptors, signaling molecules, and proteins involved in cell adhesion, RNA binding, and ion transport. Human orthologs were identified for proteins encoded by 16 of the genes. The total number of dsRNAs that we have tested for an RNAi-induced phenotype affecting the embryonic nervous system, including our previous study, is 7,312, which corresponds to approximately 50% of the genes in the Drosophila genome.
PubMed ID
PubMed Central ID
PMC1838491 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference
    Genes (21)
    Natural transposons (1)