Reference Report

Reference
Citation	Dietzl, G., Chen, D., Schnorrer, F., Su, K.C., Barinova, Y., Fellner, M., Gasser, B., Kinsey, K., Oppel, S., Scheiblauer, S., Couto, A., Marra, V., Keleman, K., Dickson, B.J. (2007). A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 448(7150): 151--156. (Export to RIS)
FlyBase ID	FBrf0200691
Publication Type	Research paper
PubMed ID	17625558
PubMed Abstract	Forward genetic screens in model organisms have provided important insights into numerous aspects of development, physiology and pathology. With the availability of complete genome sequences and the introduction of RNA-mediated gene interference (RNAi), systematic reverse genetic screens are now also possible. Until now, such genome-wide RNAi screens have mostly been restricted to cultured cells and ubiquitous gene inactivation in Caenorhabditis elegans. This powerful approach has not yet been applied in a tissue-specific manner. Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism. Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. We generated 22,270 transgenic lines, covering 88% of the predicted protein-coding genes in the Drosophila genome. Molecular and phenotypic assays indicate that the majority of these transgenes are functional. Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophila lifespan.
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Related Publication(s)
Supplementary material	Supplementary Table 1. [FBrf0202579]
Personal communication to FlyBase	RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nature
Title	Nature
Publication Year	1869-
ISBN/ISSN	0028-0836
Data from Reference
Alleles (153)
	Aats-asp^GD1339 Ace^GD1817 Actn^GD1354 AGO1^Scer\UAS.cDa Akt1^GD1361 aos^GD1666 AP-2α^GD5059 arr^GD121 arr^GD2617 arr^GD14404 baz^GD1384 bel^GD1324 bowl^GD1389 bt^GD58 bt^GD2554 bt^GD14482 cac^GD3326 Ca-P60A^GD436 cas^GD1398 Catsup^GD1635 CG11576^GD576 Cha^GD7577 cm^GD10049 Cyp309a1^GD835 Dcr-1^Scer\UAS.cDa Dcr-2^Scer\UAS.cDa Ddc^GD1189 dlg1^GD4689 DopR^GD688 ds^GD150 ds^GD2646 ds^GD14350 Dscam^GD100 Dscam^GD2596 Dscam^GD14362 ebi^GD1329 ed^GD79 ed^GD2575 ed^GD14356 Ef1α48D^GD13875 Ef1α48D^GD17848 eIF-4E^GD1432 elav^GD5206 endoB^GD14693 eya^GD1441 fliI^GD1446 fln^GD10601 flw^GD1447 flw^GD15008 Gad1^GD8508 Gpdh^GD14102 grk^GD128 grk^GD2624 grk^GD14376 Hand^GD13373 how^GD1462 Hr38^GD1463 Hsp83^GD1202 htl^GD84 htl^GD2580 htl^GD14457 if^GD1175 Ilk^GD6996 Kap3^GD7377 kirre^GD99 kirre^GD2595 kirre^GD14476 Klp64D^GD7048 lbe^GD4157 Mef2^GD5039 Mhc^GD1485 mor^GD1257 mys^GD1166 mys^GD15002 N^GD144 N^GD2640 N^GD14477 na^GD1172 nompB^GD7755 nonA^GD11253 nonA^GD15594 Nrg^GD82 Nrg^GD2578 Nrg^GD14467 nrv1^GD959 nrv2^GD960 n-syb^GD4553 n-syb^GD17382 osa^GD1502 osp^GD1504 para^GD3392 parvin^GD3687 Pfk^GD1508 Ptp69D^GD81 Ptp69D^GD2577 Ptp69D^GD14427 put^GD49 put^GD2545 put^GD14318 r2d2^Scer\UAS.cDa rhea^GD12050 rk^GD15 rk^GD2511 rk^GD14383 robo^GD112 robo^GD2608 robo^GD14414 Sb^GD473 Scer\GAL4^Act5C.PU Scer\GAL4^Bx-MS1096 Scer\GAL4^elav.PLu Scer\GAL4^ey.PH Scer\GAL4^GMR.PF Scer\GAL4^Mef2.PR Scer\GAL4^pnr-MD237 Scm^GD1526 Sema-1a^GD2504 Sema-1a^GD14307 shi^GD1529 sl^GD1535 slmo^GD14130 slou^GD4159 slpr^GD9771 sls^GD9680 Snap^GD11986 sns^GD65 sns^GD2561 stai^GD8550 Syt1^GD842 Syx5^GD1743 TfIIS^GD12773 tin^GD4155 tkv^GD53 tkv^GD2549 tkv^GD14359 Tm1^GD10529 Tm2^GD11445 tmod^GD9005 TpnC47D^GD4678 TpnC73F^GD10670 TpnC73F^GD12497 TpnC73F^GD16304 tra2^GD768 Tudor-SN^Scer\UAS.cDa twi^GD1553 unc-13^GD9877 up^GD12132 VAChT^GD3054 w^+mC W^GD1673 wb^GD1560 wupA^GD10595 zip^GD1566
Constructs (151)
	P{Act5C-GAL4.U} P{GAL4-elav.L} P{GAL4-ey.H} P{GAL4-Mef2.R} P{GAL4-ninaE.GMR} P{GD15} P{GD49} P{GD53} P{GD58} P{GD65} P{GD79} P{GD81} P{GD82} P{GD84} P{GD99} P{GD100} P{GD112} P{GD121} P{GD128} P{GD144} P{GD150} P{GD436} P{GD473} P{GD576} P{GD688} P{GD768} P{GD835} P{GD842} P{GD959} P{GD960} P{GD1166} P{GD1172} P{GD1175} P{GD1189} P{GD1202} P{GD1257} P{GD1324} P{GD1329} P{GD1339} P{GD1354} P{GD1361} P{GD1384} P{GD1389} P{GD1398} P{GD1432} P{GD1441} P{GD1446} P{GD1447} P{GD1462} P{GD1463} P{GD1485} P{GD1502} P{GD1504} P{GD1508} P{GD1526} P{GD1529} P{GD1535} P{GD1553} P{GD1560} P{GD1566} P{GD1635} P{GD1666} P{GD1673} P{GD1743} P{GD1817} P{GD2504} P{GD2511} P{GD2545} P{GD2549} P{GD2554} P{GD2561} P{GD2575} P{GD2577} P{GD2578} P{GD2580} P{GD2595} P{GD2596} P{GD2608} P{GD2617} P{GD2624} P{GD2640} P{GD2646} P{GD3054} P{GD3326} P{GD3392} P{GD3687} P{GD4155} P{GD4157} P{GD4159} P{GD4553} P{GD4678} P{GD4689} P{GD5039} P{GD5059} P{GD5206} P{GD6996} P{GD7048} P{GD7377} P{GD7577} P{GD7755} P{GD8508} P{GD8550} P{GD9005} P{GD9680} P{GD9771} P{GD9877} P{GD10049} P{GD10529} P{GD10595} P{GD10601} P{GD10670} P{GD11253} P{GD11445} P{GD11986} P{GD12050} P{GD12132} P{GD12497} P{GD12773} P{GD13373} P{GD13875} P{GD14102} P{GD14130} P{GD14318} P{GD14350} P{GD14356} P{GD14359} P{GD14362} P{GD14376} P{GD14383} P{GD14404} P{GD14414} P{GD14427} P{GD14457} P{GD14467} P{GD14476} P{GD14477} P{GD14482} P{GD14693} P{GD15002} P{GD15008} P{GD15594} P{GD16304} P{GD17382} P{GD17848} P{UAS-AGO1.D} P{UAS-Dcr-1.D} P{UAS-Dcr-2.D} P{UAS-r2d2.D} P{UAS-Tudor-SN.D} pP{MF3} pP{UAST}
Genes (109)
	Aats-asp Ace Actn AGO1 Akt1 aos AP-2α arr baz bel bowl bt cac Ca-P60A cas Catsup CG11576 Cha cm Cyp309a1 Dcr-1 Dcr-2 Ddc dlg1 DopR drl ds Dscam ebi ed Ef1α48D eIF-4E elav endoB eya fliI fln flw Gad1 Gpdh grk Hand how Hr38 Hsp83 htl if Ilk Kap3 kirre Klp64D lbe Mef2 Mhc mor mys N na nompB nonA Nrg nrv1 nrv2 n-syb osa osp para parvin Pfk Ptp69D put r2d2 rhea rk robo Sb Scer\GAL4 Scm Sema-1a shi sl slmo slou slpr sls Snap sns stai Syt1 Syx5 TfIIS tin tkv Tm1 Tm2 tmod TpnC47D TpnC73F tra2 Tudor-SN twi unc-13 up VAChT W w wb wupA zip
Insertions (3)
	P{GawB}Bx^MS1096 P{GawB}pnr^MD237 P{UAS-Dcr-2.D}1
Libraries/Collections (2)
	VDRC-1 VDRC-2
Natural transposons (1)
	P-element