Reference Report

Reference
Citation	Lucas, E.P., Raff, J.W. (2007). Maintaining the proper connection between the centrioles and the pericentriolar matrix requires Drosophila Centrosomin. J. Cell Biol. 178(5): 725--732. (Export to RIS)
FlyBase ID	FBrf0200733
Publication Type	Research paper
PubMed ID	17709428
PubMed Abstract	Centrosomes consist of two centrioles surrounded by an amorphous pericentriolar matrix (PCM), but it is unknown how centrioles and PCM are connected. We show that the centrioles in Drosophila embryos that lack the centrosomal protein Centrosomin (Cnn) can recruit PCM components but cannot maintain a proper attachment to the PCM. As a result, the centrioles "rocket" around in the embryo and often lose their connection to the nucleus in interphase and to the spindle poles in mitosis. This leads to severe mitotic defects in embryos and to errors in centriole segregation in somatic cells. The Cnn-related protein CDK5RAP2 is linked to microcephaly in humans, but cnn mutant brains are of normal size, and we observe only subtle defects in the asymmetric divisions of mutant neuroblasts. We conclude that Cnn maintains the proper connection between the centrioles and the PCM; this connection is required for accurate centriole segregation in somatic cells but is not essential for the asymmetric division of neuroblasts.
DOI	10.1083/jcb.200704081
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Alleles (9)
	cnn^f04547 cnn^HK21 Grip75^{Scer\UAS.P\T.T:Avic\GFP} rap^{T:Disc\RFP-mRFP} Sas-4^{Ubi.P.T:Avic\GFP} Scer\GAL4^69B Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} tacc^{Ubi-p63E.T:Avic\GFP-m6} αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (6)
	P{matα4-GAL-VP16} P{mRFP-Fzr} P{UASp-GFPS65C-αTub84B} P{UASp-Grip75:GFP} P{Ubi-Sas-4-GFP.P} P{Ubi-tacc.GFP}
Genes (9)
	aur cnn cp309 Grip75 rap Sas-4 Scer\GAL4 tacc αTub84B
Insertions (2)
	P{GawB}69B PBac{WH}cnn^f04547