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Citation
Behm-Ansmant, I., Gatfield, D., Rehwinkel, J., Hilgers, V., Izaurralde, E. (2007). A conserved role for cytoplasmic poly(A)-binding protein 1 (PABPC1) in nonsense-mediated mRNA decay.  EMBO J. 26(6): 1591--1601.
FlyBase ID
FBrf0200749
Publication Type
Research paper
Abstract

The nonsense-mediated mRNA decay (NMD) pathway degrades mRNAs with premature translation termination codons (PTCs). The mechanisms by which PTCs and natural stop codons are discriminated remain unclear. We show that the position of stops relative to the poly(A) tail (and thus of PABPC1) is a critical determinant for PTC definition in Drosophila melanogaster. Indeed, tethering of PABPC1 downstream of a PTC abolishes NMD. Conversely, natural stops trigger NMD when the length of the 3' UTR is increased. However, many endogenous transcripts with exceptionally long 3' UTRs escape NMD, suggesting that the increase in 3' UTR length has co-evolved with the acquisition of features that suppress NMD. We provide evidence for the existence of 3' UTRs conferring immunity to NMD. We also show that PABPC1 binding is sufficient for PTC recognition, regardless of cleavage or polyadenylation. The role of PABPC1 in NMD must go beyond that of providing positional information for PTC definition, because its depletion suppresses NMD under conditions in which translation efficiency is not affected. These findings reveal a conserved role for PABPC1 in mRNA surveillance.

PubMed ID
PubMed Central ID
PMC1829367 (PMC) (EuropePMC)
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Secondary IDs
  • FBrf0194956
Language of Publication
English
Additional Languages of Abstract
Parent Publication
Publication Type
Journal
Abbreviation
EMBO J.
Title
The EMBO Journal
Publication Year
1982-
ISBN/ISSN
0261-4189
Data From Reference
Genes (13)
Cell Lines (2)