Reference Report

Reference
Citation	Ohyama, T., Verstreken, P., Ly, C.V., Rosenmund, T., Rajan, A., Tien, A.C., Haueter, C., Schulze, K.L., Bellen, H.J. (2007). Huntingtin-interacting protein 14, a palmitoyl transferase required for exocytosis and targeting of CSP to synaptic vesicles. J. Cell Biol. 179(7): 1481--1496. (Export to RIS)
FlyBase ID	FBrf0200919
Publication Type	Research paper
PubMed ID	18158335
PubMed Abstract	Posttranslational modification through palmitoylation regulates protein localization and function. In this study, we identify a role for the Drosophila melanogaster palmitoyl transferase Huntingtin-interacting protein 14 (HIP14) in neurotransmitter release. hip14 mutants show exocytic defects at low frequency stimulation and a nearly complete loss of synaptic transmission at higher temperature. Interestingly, two exocytic components known to be palmitoylated, cysteine string protein (CSP) and SNAP25, are severely mislocalized at hip14 mutant synapses. Complementary DNA rescue and localization experiments indicate that HIP14 is required solely in the nervous system and is essential for presynaptic function. Biochemical studies indicate that HIP14 palmitoylates CSP and that CSP is not palmitoylated in hip14 mutants. Furthermore, the hip14 exocytic defects can be suppressed by targeting CSP to synaptic vesicles using a chimeric protein approach. Our data indicate that HIP14 controls neurotransmitter release by regulating the trafficking of CSP to synapses.
DOI	10.1083/jcb.200710061
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	J. Cell Biol.
Title	Journal of Cell Biology
Publication Year	1966-
ISBN/ISSN	0021-9525
Data from Reference
Aberrations (1)
	Df(3L)brm11
Alleles (17)
	Csp::n-syb^TMD.Scer\UAS Csp^2.Scer\UAS Csp^Ssp.Scer\UAS Csp^U1 Csp^X1 Hip14¹ Hip14² Hip14³ Hip14^+t6.5 Hip14^{C.T:Avic\GFP-EGFP} Hip14^{N.T:Avic\GFP-EGFP} Hip14^Scer\UAS.cOa Hip14^unspecified Scer\GAL4^elav-C155 shi¹ T:Avic\GFP^EGFP w^+mC
Constructs (7)
	P{CtermGFP-Hip14} P{Hip14^+t6.5} P{NtermGFP-Hip14} P{UAS-Csp.2} P{UAS-Csp.Ssp} P{UAS-Hip14.O} P{UAS-n-sybTMD-csp2}
Genes (16)
	brp chp Csp Csp::n-syb dlg1 Fas2 GluRIIC Hip14 lva n-syb Scer\GAL4 shi Snap Snap25 Syt1 w
Insertions (3)
	P{EPgy2}CG31739^EY12630 P{GawB}elav^C155 P{SUPor-P}Pka-C3^KG00222
Natural transposons (1)
	P-element