Reference Report

Reference
Citation	Dix, C.I., Raff, J.W. (2007). Drosophila Spd-2 recruits PCM to the sperm centriole, but is dispensable for centriole duplication. Curr. Biol. 17(20): 1759--1764. (Export to RIS)
FlyBase ID	FBrf0200963
Publication Type	Research paper
PubMed ID	17919907
PubMed Abstract	In C. elegans, genome-wide screens have identified just five essential centriole-duplication factors: SPD-2, ZYG-1, SAS-5, SAS-6, and SAS-4 [1-8]. These proteins are widely believed to comprise a conserved core duplication module [3, 9-14]. In worm embryos, SPD-2 is the most upstream component of this module, and it is also essential for pericentriolar material (PCM) recruitment to the centrioles [1, 4, 15, 16]. Here, we show that Drosophila Spd-2 (DSpd-2) is a component of both the centrioles and the PCM and has a role in recruiting PCM to the centrioles. DSpd-2 appears not, however, to be essential for centriole duplication in somatic cells. Moreover, PCM recruitment in DSpd-2 mutant somatic cells is only partially compromised, and mitosis appears unperturbed. In contrast, DSpd-2 is essential for proper PCM recruitment to the fertilizing sperm centriole, and hence for microtubule nucleation and pronuclear fusion. DSpd-2 therefore appears to have a particularly important role in recruiting PCM to the sperm centriole. We speculate that the SPD-2 family of proteins might only be absolutely essential for the recruitment of centriole duplication factors and PCM to the centriole(s) that enter the egg with the fertilizing sperm.
DOI	10.1016/j.cub.2007.08.065
Related Publication(s)
Supplementary material	Document S1. Experimental Procedures and Two Figures. [FBrf0209277]
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Curr. Biol.
Title	Current Biology
Publication Year	1991-
ISBN/ISSN	0960-9822
Data from Reference
Alleles (6)
	asl^{Ubi.T:Avic\GFP} cp309^{PACT.Ubi-p63E.T:Avic\GFP} Sas-4^{Ubi.P.T:Avic\GFP} spd-2^G20143 spd-2^{Ubi.T:Avic\GFP} αTub84B^{Scer\UAS.P\T.T:Avic\GFP-S65C}
Constructs (5)
	P{UASp-GFPS65C-αTub84B} P{Ubi-asl-GFP} P{Ubi-p63E-GFP-cp309.PACT} P{Ubi-Sas-4-GFP.P} P{Ubi-spd-2-GFP}
Genes (8)
	asl cnn cp309 His3 Sas-4 spd-2 αTub84B γTub23C
Insertions (1)
	P{EP}spd-2^G20143