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Reference Report
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| Citation | Sharma, S.K., Nirenberg, M. (2007). Silencing of genes in cultured Drosophila neurons by RNA interference. Proc. Natl. Acad. Sci. U.S.A. 104(31): 12925--12930. (Export to RIS) | ||
| FlyBase ID | FBrf0200998 | ||
| Publication Type | Research paper | ||
| PubMed ID | 17646657 | ||
| PubMed Abstract | Cultures of neuroblasts that generate abundant neurons were established from Drosophila embryos to study silencing of genes by RNA interference (RNAi). Cultured cells expressed ELAV, a marker of neurons, Futsch, a marker of neurites, and Synapsin, Synaptobrevin, and Synaptogamin, proteins involved in neurotransmitter secretion. Conditions were found for efficient transfection of cells with siRNAs for ELAV or the insulin-like receptor, which resulted in marked decreases in neurons that express ELAV and Futsch. Cells also were successfully transfected with long-chain Sox-Neuro dsRNA resulting in a 55% reduction of neurons expressing Futsch. The results suggest that this cultured neural cell system can be used to study RNAi-dependent silencing of genes involved in many kinds of neural functions. | ||
| DOI | 10.1073/pnas.0704299104 | ||
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| Language of Publication | English | ||
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| Publication Type | Journal | ||
| Abbreviation | Proc. Natl. Acad. Sci. U.S.A. | ||
| Title | Proceedings of the National Academy of Sciences of the United States of America | ||
| Publication Year | 1915- | ||
| ISBN/ISSN | 0027-8424 | ||
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Data from Reference
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| Genes (5)
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