Open Close
Reference
Citation
Pane, A., Wehr, K., Sch├╝pbach, T. (2007). zucchini and squash encode two putative nucleases required for rasiRNA production in the Drosophila germline.  Dev. Cell 12(6): 851--862.
FlyBase ID
FBrf0201231
Publication Type
Research paper
Abstract

RNAi is a widespread mechanism by which organisms regulate gene expression and defend their genomes against viruses and transposable elements. Here we report the identification of Drosophila zucchini (zuc) and squash (squ), which function in germline RNAi processes. Zuc and Squ contain domains with homologies to nucleases. Mutant females are sterile and show dorsoventral patterning defects during oogenesis. In addition, Oskar protein is ectopically expressed in early oocytes, where it is normally silenced by RNAi mechanisms. Zuc and Squ localize to the perinuclear nuage and interact with Aubergine, a PIWI class protein. Mutations in zuc and squ induce the upregulation of Het-A and Tart, two telomere-specific transposable elements, and the expression of Stellate protein in the Drosophila germline. We show that these defects are due to the inability of zuc and squ mutants to produce repeat-associated small interfering RNAs.

PubMed ID
PubMed Central ID
PMC1945814 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference
    Aberrations (5)
    Alleles (18)
    Genes (15)
    Physical Interactions (2)
    Natural transposons (4)
    Experimental Tools (3)
    Transgenic Constructs (5)