Reference Report

Reference
Citation	Pack-Chung, E., Kurshan, P.T., Dickman, D.K., Schwarz, T.L. (2007). A Drosophila kinesin required for synaptic bouton formation and synaptic vesicle transport. Nat. Neurosci. 10(8): 980--989. (Export to RIS)
FlyBase ID	FBrf0201241
Publication Type	Research paper
PubMed ID	17643120
PubMed Abstract	The morphological transition of growth cones to synaptic boutons characterizes synaptogenesis. Here we have isolated mutations in immaculate connections (imac; CG8566), a previously uncharacterized Drosophila gene encoding a member of the Kinesin-3 family. Whereas earlier studies in Drosophila implicated Kinesin-1 in transporting synaptic vesicle precursors, we find that Imac is essential for this transport. An unexpected feature of imac mutants is the failure of synaptic boutons to form. Motor neurons lacking imac properly target to muscles but remain within target fields as thin processes, a structure that is distinct from either growth cones or mature terminals. Few active zones form at these endings. We show that the arrest of synaptogenesis is not a secondary consequence of the absence of transmission. Our data thus indicate that Imac transports components required for synaptic maturation and provide insight into presynaptic maturation as a process that can be differentiated from axon outgrowth and targeting.
DOI	10.1038/nn1936
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Nat. Neurosci.
Title	Nature Neuroscience
Publication Year	1998-
ISBN/ISSN	1097-6256
Data from Reference
Aberrations (1)
	Df(2R)Alk-21
Alleles (17)
	Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} Rnor\ANF^{Scer\UAS.T:Avic\GFP-EMD} Scer\FLP1^Scer\UAS.cDa Scer\GAL4^da.G32 Scer\GAL4^elav-C155 Scer\GAL4^ey.PH Syx1A^Δ229 unc-104⁵⁰ unc-104⁵² unc-104⁷⁶ unc-104¹⁰² unc-104¹¹⁶ unc-104¹⁶⁰ unc-104¹⁷⁰ unc-104¹⁷² unc-104^{Scer\UAS.cPCa} W^GMR.PG
Constructs (7)
	P{GAL4-da.G32} P{GAL4-ey.H} P{GMR-hid} P{UAS-FLP1.D} P{UAS-imac.PC} P{UAS-mCD8::GFP.L} P{UAS-preproANF-EMD}
Genes (16)
	brp Fas2 futsch GluRIIA GluRIIC Khc lap Mmus\Cd8a Rnor\ANF Scer\FLP1 Scer\GAL4 Syt1 Syx1A unc-104 VGlut W
Insertions (1)
	P{GawB}elav^C155
Natural transposons (1)
	P-element