Reference Report

Reference
Citation	Chan, C.C., Zhang, S., Cagatay, T., Wharton, K.A. (2007). Cell-autonomous, myristyl-independent activity of the Drosophila Wnt/Wingless antagonist Naked cuticle (Nkd). Dev. Biol. 311(2): 538--553. (Export to RIS)
FlyBase ID	FBrf0201330
Publication Type	Research paper
PubMed ID	17942091
PubMed Abstract	Robust animal development, tissue homeostasis, and stem cell renewal requires precise control of the Wnt/beta-catenin signaling axis. In the embryo of the fruit fly Drosophila melanogaster, the naked cuticle (nkd) gene attenuates signaling by the Wnt ligand Wingless (Wg) during segmentation. nkd mutants have been reported to exhibit abnormalities in wg transcription, Wg protein distribution and/or transport, and the intracellular response to Wg, but the relationship between each alteration and the molecular mechanism of Nkd action remains unclear. In addition, whether Nkd acts in a cell-autonomous or nonautonomous fashion in the embryo is not known. Mammalian Nkd homologs have N-terminal consensus sequences that direct the post-translational addition of a lipophilic myristoyl moiety, but fly and mosquito Nkd, while sharing N-terminal sequence homology, lack a myristoylation consensus sequence. Here we provide evidence that fly Nkd acts cell-autonomously in the embryo, with its N-terminus able to confer unique functional properties and membrane association that cannot be mimicked in vivo by heterologous myristoylation consensus sequences. In conjunction with our recent observation that Nkd requires nuclear localization for function, our data suggest that Nkd acts at more than one subcellular location within signal-receiving cells to attenuate Wg signaling.
DOI	10.1016/j.ydbio.2007.09.002
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Dev. Biol.
Title	Developmental Biology
Publication Year	1959-
ISBN/ISSN	0012-1606
Data from Reference
Alleles (29)
	Avic\GFP^{E.Scer\UAS.T:Myr-Src64B} Avic\GFP^Scer\UAS.cYa Axn^{Scer\UAS.T:Avic\GFP} Ecol\lacZ^Scer\UAS.cBa Mmus\Nkd1^{Scer\UAS.T:Avic\GFP-EGFP} nkd::Mmus\Nkd1^{f30aa.Scer\UAS.T:Avic\GFP-EGFP} nkd::Mmus\Nkd1^{fNf30aa.Scer\UAS.T:Avic\GFP-EGFP} nkd::Mmus\Nkd1^{m1NΔN.Scer\UAS.T:Avic\GFP} nkd³ nkd^{7H.Scer\UAS.T:Avic\GFP-EGFP} nkd^{GFPN.Scer\UAS.T:Avic\GFP-EGFP,T:Hsap\MYC} nkd^{GFPX.Scer\UAS.T:Avic\GFP-EGFP,T:Hsap\MYC} nkd^{NBg.Scer\UAS.T:Avic\GFP-EGFP} nkd^{NBg.Scer\UAS.T:Myr-Src64B,T:Avic\GFP-EGFP} nkd^{NBg.Scer\UAS.T:Myr-Src64B-GA,T:Avic\GFP-EGFP} nkd^Scer\UAS.cZa nkd^{Scer\UAS.T:Avic\GFP-EGFP} nkd^{SrcRBg.Scer\UAS.T:Myr-Src64B,T:Avic\GFP-EGFP} nkd^{ΔN.GFPC.Scer\UAS.T:Avic\GFP} pan^ΔN.Scer\UAS Scer\GAL4^71B Scer\GAL4^B119 Scer\GAL4^da.G32 Scer\GAL4^en-e16E Scer\GAL4^prd.RG1 Scer\GAL4^ptc-559.1 T:Avic\GFP^EGFP T:Myr-Src64B^GA w^+mC
Constructs (21)
	P{GAL4-da.G32} P{GAL4-prd.F} P{UAS-Axn.GFP} P{UAS-GFP.Y} P{UAS-lacZ.B} P{UAS-Mmus\Nkd1.GFPC} P{UAS-mNkd1.fNf30aa.GFPC} P{UAS-nkd::Mmus\Nkd1.f30aa.GFPC} P{UAS-nkd.7H.GFPC} P{UAS-nkd.GFPC} P{UAS-nkd.GFPN.mycC} P{UAS-nkd.GFPX.mycC} P{UAS-nkd.m1NΔN.GFPC} P{UAS-nkd.NBg.GFPC} P{UAS-nkd.SrcG2ANBg.GFPC} P{UAS-nkd.SrcNBg.GFPC} P{UAS-nkd.SrcRBg.GFPC} P{UAS-nkd.Z} P{UAS-nkd.ΔN.GFPC} P{UAS-pan.dTCFΔN} P{UAS-Src1-89EGFP}
Genes (15)
	arm Avic\GFP Axn Ecol\lacZ en Mmus\Nkd1 nkd nkd::Mmus\Nkd1 pan Scer\GAL4 T:Avic\GFP T:Hsap\MYC T:Myr-Src64B w wg
Insertions (4)
	P{en2.4-GAL4}e16E P{GawB}71B P{GawB}B119 P{GawB}ptc^559.1
Natural transposons (1)
	P-element