Reference Report

Reference
Citation	Bai, X., Larschan, E., Kwon, S.Y., Badenhorst, P., Kuroda, M.I. (2007). Regional control of chromatin organization by noncoding roX RNAs and the NURF remodeling complex in Drosophila melanogaster. Genetics 176(3): 1491--1499. (Export to RIS)
FlyBase ID	FBrf0201334
Publication Type	Research paper
PubMed ID	17507677
PubMed Abstract	Dosage compensation in Drosophila is mediated by a histone-modifying complex that upregulates transcription of genes on the single male X chromosome. The male-specific lethal (MSL) complex contains at least five proteins and two noncoding roX (RNA on X) RNAs. The mechanism by which the MSL complex targets the X chromosome is not understood. Here we use a sensitized system to examine the function of roX genes on the X chromosome. In mutants that lack the NURF nucleosome remodeling complex, the male polytene X chromosome is severely distorted, appearing decondensed. This aberrant morphology is dependent on the MSL complex. Strikingly, roX mutations suppress the Nurf mutant phenotype regionally on the male X chromosome. Furthermore, a roX transgene induces disruption of local flanking autosomal chromatin in Nurf mutants. Taken together, these results demonstrate the potent capability of roX genes to organize large chromatin domains in cis, on the X chromosome. In addition to interacting functions at the level of chromosome morphology, we also find that NURF complex and MSL proteins have opposing effects on roX RNA transcription. Together, these results demonstrate the importance of a local balance between modifying activities that promote and antagonize chromatin compaction within defined chromatin domains in higher organisms.
DOI	10.1534/genetics.107.071571
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Language of Publication	English
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Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (2)
	Df(1)52 Df(3L)3643
Alleles (14)
	Avic\GFP^E.roX1.DHS Avic\GFP^E.roX1 Avic\GFP^roX2.DHS Avic\GFP^roX2.PB E(bx)^Nurf301-2 E(bx)^Nurf301-3 E(bx)^Nurf301-4 E(bx)^Nurf301-5 E(bx)^Nurf301-10 E(bx)^Nurf301-12 Iswi^unspecified roX1^ex6 roX2^t4.0 RpII215^+tcos4Δ4.3
Constructs (6)
	P{GMroX2} P{roX1-EGFP.B} P{roX1-EGFP.DHS} P{roX2-GFP.B} P{roX2-GFP.DHS} P{w⁺4Δ4.3}
Genes (22)
	Asx Avic\GFP E(bx) Gs2 Iswi msl-1 msl-2 Pc Pcl Pgd piwi pr-set7 Psc roX1 roX2 RpII215 Scm Su(var)3-9 Su(var)205 Su(z)2 Trl Zw
Insertions (6)
	P{roX1-EGFP.B}1 P{roX1-EGFP.B}5 P{roX1-EGFP.DHS}5 P{roX1-EGFP.DHS}6 P{roX2-GFP.B}5.4 P{roX2-GFP.DHS}2.3
Natural transposons (1)
	P-element