Reference Report

Reference
Citation	Wei, D.S., Rong, Y.S. (2007). A genetic screen for DNA double-strand break repair mutations in Drosophila. Genetics 177(1): 63--77. (Export to RIS)
FlyBase ID	FBrf0201349
Publication Type	Research paper
PubMed ID	17660539
PubMed Abstract	The study of DNA double-strand break (DSB) repair has been greatly facilitated by the use of rare-cutting endonucleases, which induce a break precisely at their cut sites that can be strategically placed in the genome. We previously established such a system in Drosophila and showed that the yeast I-SceI enzyme cuts efficiently in Drosophila cells and those breaks are effectively repaired by conserved mechanisms. In this study, we determined the genetic requirements for the repair of this I-SceI-induced DSB in the germline. We show that Drosophila Rad51 and Rad54 are both required for homologous repair by gene conversion, but are dispensable for single-strand annealing repair. We provided evidence suggesting that Rad51 is more stringently required than Rad54 for intersister gene conversion. We uncovered a significant role of DNA ligase IV in nonhomologous end joining. We conducted a screen for candidate mutations affecting DSB repair and discovered novel mutations in genes that include mutagen sensitive 206, single-strand annealing reducer, and others. In addition, we demonstrated an intricate balance among different repair pathways in which the cell differentially utilizes repair mechanisms in response to both changes in the genomic environment surrounding the break and deficiencies in one or the other repair pathways.
DOI	10.1534/genetics.107.077693
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Secondary IDs
Language of Publication	English
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Parent Publication
Publication Type	Journal
Abbreviation	Genetics
Title	Genetics
Publication Year	1916-
ISBN/ISSN	0016-6731
Data from Reference
Aberrations (4)
	Df(2L)JS17 Df(3L)Aprt-1 Df(3R)sbd105 Df(3R)X3F
Alleles (50)
	Blm^D2 Blm^D3 Gen^Z4325 Gen^Z5997 Lig4¹¹ mei-9^A2 mei-41^29D mei-41^A2 mio² mu2¹ mus101^D1 mus101^D2 mus102^D1 mus105^A1 mus109^D1 mus109^D2 mus112^RT2 mus114^RT1 mus201^D1 mus206^A1 mus207^A1 mus209²⁷³⁵ mus209^B1 mus301^D1 mus301^D4 mus304^D1 mus304^D3 mus305^D1 mus305^D2 mus306^D1 mus307^D1 mus308^D2 mus311^D1 mus311^D2 mus312^D1 mus312^D2 mus327^ZIII-5906 okr^AA okr^RU okr^WS rad201¹ Sir2^2A-7-11 spn-A¹ spn-B¹ spn-D¹ spn-E¹ ssar¹ w^{wIw.8z.Scer\FRT} w^{wIw.yellow.Scer\FRT} w^wIw
Constructs (3)
	P{wIw.FRT.8z} P{wIw.FRT.yellow} P{wIw.FRT}
Genes (36)
	Blm cu Gen Lig4 mei-9 mei-41 mio mu2 mus101 mus102 mus105 mus109 mus112 mus114 mus201 mus206 mus207 mus209 mus301 mus304 mus305 mus306 mus307 mus308 mus311 mus312 mus327 okr rad201 Sir2 spn-A spn-B spn-D spn-E ssar w
Natural transposons (1)
	P-element