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Citation
Horiuchi, D., Collins, C.A., Bhat, P., Barkus, R.V., DiAntonio, A., Saxton, W.M. (2007). Control of a kinesin-cargo linkage mechanism by JNK pathway kinases.  Curr. Biol. 17(15): 1313--1317.
FlyBase ID
FBrf0201414
Publication Type
Research paper
Abstract
Long-distance organelle transport toward axon terminals, critical for neuron development and function, is driven along microtubules by kinesins [1, 2]. The biophysics of force production by various kinesins is known in detail. However, the mechanisms of in vivo transport processes are poorly understood because little is known about how motor-cargo linkages are controlled. A c-Jun N-terminal kinase (JNK)-interacting protein (JIP1) has been identified previously as a linker between kinesin-1 and certain vesicle membrane proteins, such as Alzheimer's APP protein and a reelin receptor ApoER2 [3, 4]. JIPs are also known to be scaffolding proteins for JNK pathway kinases [5, 6]. Here, we report evidence that a Drosophila ubiquitin-specific hydrolase and a JNK signaling pathway that it modulates can regulate a JIP1-kinesin linkage. The JNK pathway includes a MAPKKK (Wallenda/DLK), a MAPKK (Hemipterous/MKK7), and the Drosophila JNK homolog Basket. Genetic tests indicate that those kinases are required for normal axonal transport. Biochemical tests show that activation of Wallenda (DLK) and Hemipterous (MKK7) disrupts binding between kinesin-1 and APLIP1, which is the Drosophila JIP1 homolog. This suggests a control mechanism in which an activated JNK pathway influences axonal transport by functioning as a kinesin-cargo dissociation factor.
PubMed ID
PubMed Central ID
PMC2041807 (PMC) (EuropePMC)
Related Publication(s)
Note
Motor proteins: trafficking and signaling collide.
Verhey, 2007, Curr. Biol. 17(18): R804--R806 [FBrf0216114]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference
    Genes (6)
    Physical Interactions (3)
    Cell Lines (1)