Reference Report

Reference
Citation	Okamura, T., Shimizu, H., Nagao, T., Ueda, R., Ishii, S. (2007). ATF-2 regulates fat metabolism in Drosophila. Mol. Biol. Cell 18(4): 1519--1529. (Export to RIS)
FlyBase ID	FBrf0201502
Publication Type	Research paper
PubMed ID	17314398
PubMed Abstract	ATF-2 is a member of the ATF/CREB family of transcription factors that is activated by stress-activated protein kinases such as p38. To analyze the physiological role of Drosophila ATF-2 (dATF-2), we generated dATF-2 knockdown flies using RNA interference. Reduced dATF-2 in the fat body, the fly equivalent of the mammalian liver and adipose tissue, decreased survival under starvation conditions. This was due to smaller triglyceride reserves of dATF-2 knockdown flies than control flies. Among multiple genes that control triglyceride levels, expression of the Drosophila PEPCK (dPEPCK) gene was strikingly reduced in dATF-2 knockdown flies. PEPCK is a key enzyme for both gluconeogenesis and glyceroneogenesis, which is a pathway required for triglyceride synthesis via glycerol-3-phosphate. Although the blood sugar level in dATF-2 knockdown flies was almost same as that in control flies, the activity of glyceroneogenesis was reduced in the fat bodies of dATF-2 knockdown flies. Thus, reduced glyceroneogenesis may at least partly contribute to decreased triglyceride stores in the dATF-2 knockdown flies. Furthermore we showed that dATF-2 positively regulated dPEPCK gene transcription via several CRE half-sites in the PEPCK promoter. Thus, dATF-2 is critical for regulation of fat metabolism.
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Secondary IDs	FBrf0195111
Language of Publication	English
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Publication Type	Journal
Abbreviation	Mol. Biol. Cell
Title	Molecular Biology of the Cell
Publication Year	1992-
ISBN/ISSN	1059-1524
Data from Reference
Alleles (7)
	Atf-2^+t13.6 Atf-2^{dsRNA.Scer\UAS.cOa} Scer\GAL4^Act.PU Scer\GAL4^c564 Scer\GAL4^da.G32 Scer\GAL4^Mhc.PW w^+mC
Constructs (5)
	P{Act-GAL4.U} P{Atf-2^+t13.6} P{GAL4-da.G32} P{GAL4-Mhc.W} P{UAS-Atf-2.RNAi.O}
Genes (9)
	ACC AcCoAS Atf-2 CG3523 CG15859 CG16896 Pepck Scer\GAL4 w
Insertions (1)
	P{GawB}c564
Natural transposons (1)
	P-element