Reference Report

Reference
Citation	Goodliffe, J.M., Cole, M.D., Wieschaus, E. (2007). Coordinated regulation of Myc trans-activation targets by polycomb and the trithorax group protein Ash1. BMC Mol. Biol. 8(): 40. (Export to RIS)
FlyBase ID	FBrf0201535
Publication Type	Research paper
PubMed ID	17519021
PubMed Abstract	The Myc oncoprotein is a transcriptional regulator whose function is essential for normal development. Myc is capable of binding to 10% of the mammalian genome, and it is unclear how a developing embryo controls the DNA binding of its abundant Myc proteins in order to avoid Myc's potential for inducing tumorigenesis.To identify chromatin binding proteins with a potential role in controlling Myc activity, we established a genetic assay for dMyc activity in Drosophila. We conducted a genome-wide screen using this assay, and identified the Trithorax Group protein Ash1 as a modifier of dMyc activity. Ash1 is a histone methyltransferase known for its role in opposing repression by Polycomb. Using RNAi in the embryo and Affymetrix microarrays, we show that ash1 RNAi causes the increased expression of many genes, suggesting that it is directly or indirectly required for repression in the embryo, in contrast to its known role in maintenance of activation. Many of these genes also respond similarly upon depletion of Pc and pho transcripts, as determined by concurrent microarray analysis of Pc and pho RNAi embryos, suggesting that the three are required for low levels of expression of a common set of targets. Further, many of these overlapping targets are also activated by Myc overexpression. We identify a second group of genes whose expression in the embryo requires Ash1, consistent with its previously established role in maintenance of activation. We find that this second group of Ash1 targets overlaps those activated by Myc and that ectopic Myc overcomes their requirement for Ash1.Genetic, genomic and chromatin immunoprecipitation data suggest a model in which Pc, Ash1 and Pho are required to maintain a low level of expression of embryonic targets of activation by Myc, and that this occurs, directly or indirectly, by a combination of disparate chromatin modifications.
DOI	10.1186/1471-2199-8-40
Related Publication(s)
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Associated Information
Comments
Associated Files
Other Information
Secondary IDs	FBrf0200379
Language of Publication	English
Additional Languages of Abstract
Also Published As
Parent Publication
Publication Type	Journal
Abbreviation	BMC Mol. Biol.
Title	BMC Molecular Biology
Publication Year	2000-
ISBN/ISSN	1471-2199
Data from Reference
Aberrations (11)
	Df(2R)vg135 Df(3L)Aprt-32 Df(3L)BSC10 Df(3L)BSC13 Df(3L)fz-CAL5 Df(3L)kto2 Df(3L)lxd6 Df(3L)XS533 Df(3R)M-Kx1 Df(3R)p712 Df(3R)T-32
Alleles (13)
	ash1⁴ ash1^dsRNA.cGa dm^BG00605 dm^BG02383 dm^Scer\UAS.cZa E(z)⁵ E(z)⁶¹ Pc³ Pc^dsRNA.cGa pho¹ pho^dsRNA.cGa Psc¹ Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}
Constructs (2)
	P{matα4-GAL-VP16} P{UAS-dm.Z}
Genes (18)
	Act88F ash1 Atg8a Ccp84Ac CG16712 Cyp309a1 Dhc62B dm E(z) fs(1)N His3 IM1 Lip1 Pc pho Psc Scer\GAL4 αTub67C
Insertions (1)
	P{GT1}BG00979